Hepatitis A synthetic peptide VP3(110-121) miscibility with dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylglycerol, and stearylamine monolayers

P. Sospedra, M. A. Alsina, M. Espina, F. Reig, I. Haro, C. Mestres

Research output: Indexed journal article Articlepeer-review

4 Citations (Scopus)

Abstract

To prepare liposomes containing a synthetic hepatitis A virus antigen (HAV) [VP3(110-121)] as a vaccine, the miscibility of this peptide (with negative net charge) with a neutral lipid [dipalmitoylphosphatidylcholine (DPPC)], a negatively charged lipid [dipalmitoylphosphatidylglycerol (DPPG)], and a positively charged lipid [Stearylamine (SA)] was studied through compression isotherms of monolayers. Mixtures with DPPC and SA showed a low degree of interaction with the peptide, the composition of the monolayer being stable through compression. For DPPG-containing monolayers larger positive deviations from ideality were found, and the peptide was squeezed out from the monolayer at a DPPG/VP3(110-121) mole fraction of 0.8/0.2. All this suggests that besides hydrophobic interactions between the peptide and the lipid, electrostatic forces also play a role; thus it seems that neutral and positively charged lipids would be more suitable for preparing stable liposomes with VP3(110-121). (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalJournal of Colloid and Interface Science
Volume221
Issue number2
DOIs
Publication statusPublished - 15 Jan 2000
Externally publishedYes

Keywords

  • Hepatitis A
  • Lipid monolayers
  • Lipid-peptide interaction
  • Synthetic vaccine

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