HEK293 cell-based bioprocess development at bench scale by means of online monitoring in shake flasks (RAMOS and SFR)

Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina

Research output: Book chapterChapterpeer-review

4 Citations (Scopus)

Abstract

The platforms for bioprocess development have been developed in parallel to the needs of the manufacturing industry of biopharmaceuticals, aiming to ensure the quality and safety of their products. In this sense, Quality by Design (QbD) and Process Analytical Technology (PAT) have become the pillars for quality control and quality assurance. A new combination of Shake Flask Reader (SFR) and Respiration Activity Monitoring System for online determination of OTR and CTR (RAMOS) allows online monitoring of main culture parameters needed for bioprocess development (pH, pO2, OTR, CTR, and QR) as presented below. Eventually, a case study of the application of the combination of SFR-RAMOS system is presented. The case study discloses the optimization of HEK293 cells cultures through the manipulation of their metabolic behavior.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-103
Number of pages21
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2095
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bioprocess optimization
  • Culture monitoring
  • Glucose and lactate co-consumption
  • HEK293 cells
  • Metabolic phases
  • OTR
  • Ramos
  • SFR

Fingerprint

Dive into the research topics of 'HEK293 cell-based bioprocess development at bench scale by means of online monitoring in shake flasks (RAMOS and SFR)'. Together they form a unique fingerprint.

Cite this