Fibrinogen nitrotyrosination after ischemic stroke impairs thrombolysis and promotes neuronal death

Gerard ILL-Raga, Ernest Palomer, Eva Ramos-Fernández, Francesc X. Guix, Mònica Bosch-Morató, Biuse Guivernau, Marta Tajes, Victòria Valls-Comamala, Jordi Jiménez-Conde, Angel Ois, Fernando Pérez-Asensio, Mario Reyes-Navarro, Carolina Caballo, Gabriel Gil-Gómez, Irene Lopez-Vilchez, Ana M. Galan, Francesc Alameda, Gines Escolar, Carlos Opazo, Anna M. PlanasJaume Roquer, Miguel A. Valverde, Francisco J. Muñoz

Research output: Indexed journal article Articlepeer-review

23 Citations (Scopus)

Abstract

Ischemic stroke is an acute vascular event that compromises neuronal viability, and identification of the pathophysiological mechanisms is critical for its correct management. Ischemia produces increased nitric oxide synthesis to recover blood flow but also induces a free radical burst. Nitric oxide and superoxide anion react to generate peroxynitrite that nitrates tyrosines. We found that fibrinogen nitrotyrosination was detected in plasma after the initiation of ischemic stroke in human patients. Electron microscopy and protein intrinsic fluorescence showed that in vitro nitrotyrosination of fibrinogen affected its structure. Thromboelastography showed that initially fibrinogen nitrotyrosination retarded clot formation but later made the clot more resistant to fibrinolysis. This result was independent of any effect on thrombin production. Immunofluorescence analysis of affected human brain areas also showed that both fibrinogen and nitrotyrosinated fibrinogen spread into the brain parenchyma after ischemic stroke. Therefore, we assayed the toxicity of fibrinogen and nitrotyrosinated fibrinogen in a human neuroblastoma cell line. For that purpose we measured the activity of caspase-3, a key enzyme in the apoptotic pathway, and cell survival. We found that nitrotyrosinated fibrinogen induced higher activation of caspase 3. Accordingly, cell survival assays showed a more neurotoxic effect of nitrotyrosinated fibrinogen at all concentrations tested. In summary, nitrotyrosinated fibrinogen would be of pathophysiological interest in ischemic stroke due to both its impact on hemostasis - it impairs thrombolysis, the main target in stroke treatments - and its neurotoxicity that would contribute to the death of the brain tissue surrounding the infarcted area.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1852
Issue number3
DOIs
Publication statusPublished - 1 Mar 2015
Externally publishedYes

Keywords

  • Apoptosis
  • Fibrinogen
  • Ischemic stroke
  • Nitric oxide
  • Peroxynitrite

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