Fast-tracking of single emitters in large volumes with nanometer precision

BORIS LOUIS; RAFAEL CAMACHO; ROGER BRESOLÍ-OBACH; SERGEY ABAKUMOV; JOHANNES VANDAELE; TESTSUHIRO KUDO; HIROSHI MASUHARA; IVAN G. SCHEBLYKIN; JOHAN HOFKENS; SUSANA ROCHA

doi:10.1364/OE.401557

Fast-tracking of single emitters in large volumes with nanometer precision

BORIS LOUIS, RAFAEL CAMACHO, ROGER BRESOLÍ-OBACH, SERGEY ABAKUMOV, JOHANNES VANDAELE, TESTSUHIRO KUDO, HIROSHI MASUHARA, IVAN G. SCHEBLYKIN, JOHAN HOFKENS, SUSANA ROCHA

Research output: Indexed journal article › Article › peer-review

29 Citations (Scopus)

Abstract

Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 μm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 μm fluorescent beads into an optical trap.

Original language	English
Article number	401557
Pages (from-to)	28656-28671
Number of pages	16
Journal	Optics Express
Volume	28
Issue number	19
DOIs	https://doi.org/10.1364/OE.401557
Publication status	Published - Sept 2020

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abstract = "Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 μm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 μm fluorescent beads into an optical trap.",

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AU - LOUIS, BORIS

AU - CAMACHO, RAFAEL

AU - BRESOLÍ-OBACH, ROGER

AU - ABAKUMOV, SERGEY

AU - VANDAELE, JOHANNES

AU - KUDO, TESTSUHIRO

AU - MASUHARA, HIROSHI

AU - SCHEBLYKIN, IVAN G.

AU - HOFKENS, JOHAN

AU - ROCHA, SUSANA

PY - 2020/9

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Fast-tracking of single emitters in large volumes with nanometer precision

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