TY - JOUR
T1 - Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro
AU - Almansa, Daniel
AU - Peinado, Héctor
AU - García‐rodríguez, Raquel
AU - Casadomé‐perales, Álvaro
AU - Dotti, Carlos G.
AU - Guix, Francesc X.
N1 - Funding Information:
Funding: This work was partially supported by grant SAF 2019: 104389RB‐I00 from the Spanish Ministry of Science and Innovation, State Agency of Investigation (AEI) and EU FEDER funds (C.G.D) and by the European Commission– NextGenerationEU CSIC’s Thematic Platforms (PTI+ Neuro‐Aging) (C.G.D). DA was awarded a “Ayuda para el Fomento de la Investigación en Estudios de Máster‐UAM” grant.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Extracellular vesicles (EVs) play an important role in intercellular communication and are involved in both physiological and pathological processes. In the central nervous system (CNS), EVs secreted from different brain cell types exert a sundry of functions, from modulation of astrocytic proliferation and microglial activation to neuronal protection and regeneration. However, the effect of aging on the biological functions of neural EVs is poorly understood. In this work, we studied the biological effects of small EVs (sEVs) isolated from neural cells maintained for 14 or 21 days in vitro (DIV). We found that EVs isolated from 14 DIV cultures reduced the extracellular levels of lactate dehydrogenase (LDH), the expression levels of the astrocytic protein GFAP, and the complexity of astrocyte architecture suggesting a role in lowering the reactivity of astrocytes, while EVs produced by 21 DIV cells did not show any of the above effects. These results in an in vitro model pave the way to evaluate whether similar results occur in vivo and through what mechanisms.
AB - Extracellular vesicles (EVs) play an important role in intercellular communication and are involved in both physiological and pathological processes. In the central nervous system (CNS), EVs secreted from different brain cell types exert a sundry of functions, from modulation of astrocytic proliferation and microglial activation to neuronal protection and regeneration. However, the effect of aging on the biological functions of neural EVs is poorly understood. In this work, we studied the biological effects of small EVs (sEVs) isolated from neural cells maintained for 14 or 21 days in vitro (DIV). We found that EVs isolated from 14 DIV cultures reduced the extracellular levels of lactate dehydrogenase (LDH), the expression levels of the astrocytic protein GFAP, and the complexity of astrocyte architecture suggesting a role in lowering the reactivity of astrocytes, while EVs produced by 21 DIV cells did not show any of the above effects. These results in an in vitro model pave the way to evaluate whether similar results occur in vivo and through what mechanisms.
KW - Aging
KW - Astrocytes
KW - Extracellular vesicles (EVs)
KW - Glial fibrillary acidic protein (GFAP)
KW - Neural cultures
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UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000757557400001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.3390/ijms23031371
DO - 10.3390/ijms23031371
M3 - Article
C2 - 35163295
AN - SCOPUS:85123378277
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 3
M1 - 1371
ER -