Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro

Daniel Almansa, Héctor Peinado, Raquel García‐rodríguez, Álvaro Casadomé‐perales, Carlos G. Dotti, Francesc X. Guix

Research output: Indexed journal article Articlepeer-review

2 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) play an important role in intercellular communication and are involved in both physiological and pathological processes. In the central nervous system (CNS), EVs secreted from different brain cell types exert a sundry of functions, from modulation of astrocytic proliferation and microglial activation to neuronal protection and regeneration. However, the effect of aging on the biological functions of neural EVs is poorly understood. In this work, we studied the biological effects of small EVs (sEVs) isolated from neural cells maintained for 14 or 21 days in vitro (DIV). We found that EVs isolated from 14 DIV cultures reduced the extracellular levels of lactate dehydrogenase (LDH), the expression levels of the astrocytic protein GFAP, and the complexity of astrocyte architecture suggesting a role in lowering the reactivity of astrocytes, while EVs produced by 21 DIV cells did not show any of the above effects. These results in an in vitro model pave the way to evaluate whether similar results occur in vivo and through what mechanisms.

Original languageEnglish
Article number1371
JournalInternational Journal of Molecular Sciences
Volume23
Issue number3
DOIs
Publication statusPublished - 1 Feb 2022

Keywords

  • Aging
  • Astrocytes
  • Extracellular vesicles (EVs)
  • Glial fibrillary acidic protein (GFAP)
  • Neural cultures

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