Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase

Josep Lluís Viladot; Bruce Stone; Hugues Driguez; Antoni Planas

doi:10.1016/S0008-6215(98)00212-2

Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase

Josep Lluís Viladot
, Bruce Stone
, Hugues Driguez
, Antoni Planas^*

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

20 Citations (Scopus)

Abstract

Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20% yield. Copyright (C) 1997 Elsevier Science Ltd.

Original language	English
Pages (from-to)	95-99
Number of pages	5
Journal	Carbohydrate Research
Volume	311
Issue number	1-2
DOIs	https://doi.org/10.1016/S0008-6215(98)00212-2
Publication status	Published - Sept 1998

Keywords

Enzymatic synthesis
Glycosyl fluoride
Hexasaccharide
Transglycosylation; (1→3),(1→4)-β-glucanase

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10.1016/S0008-6215(98)00212-2

Cite this

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title = "Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase",

abstract = "Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49\% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20\% yield. Copyright (C) 1997 Elsevier Science Ltd.",

keywords = "Enzymatic synthesis, Glycosyl fluoride, Hexasaccharide, Transglycosylation; (1→3),(1→4)-β-glucanase",

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AU - Viladot, Josep Lluís

AU - Stone, Bruce

AU - Driguez, Hugues

AU - Planas, Antoni

PY - 1998/9

Y1 - 1998/9

N2 - Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20% yield. Copyright (C) 1997 Elsevier Science Ltd.

AB - Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20% yield. Copyright (C) 1997 Elsevier Science Ltd.

KW - Enzymatic synthesis

KW - Glycosyl fluoride

KW - Hexasaccharide

KW - Transglycosylation; (1→3),(1→4)-β-glucanase

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Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase

Abstract

Keywords

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