Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector

Meritxell Ventura; Montserrat Agut; Francesc Broto-Puig; Lluís Comellas

Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector

Meritxell Ventura, Montserrat Agut, Francesc Broto-Puig, Lluís Comellas

Research output: Indexed journal article › Article › peer-review

3 Citations (Scopus)

Abstract

A new, fast and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of ochratoxin A (OTA) production by fungi in culture media. The sample preparation consists of one step solid-phase extraction using a polymeric sorbent. The HPLC analysis uses a narrow-bore reversed-phase C18 column and an isocratic mobile phase composed of acetonitrile + water (0.1% HCOOH) (40+60) and fluorescence detector. The identity of this toxin was confirmed using electrospray ionization mass spectrometric detection (ESI/MS) in the positive ionization mode. The method had a recovery of 89.7% and a precision of 4.7% (n=6) for a sample which contains 17.3 ng/ml of OTA in culture media. The performance of the polymeric sorbent tested is as good as other studies published in the analysis of OTA. The detection limit of the new method is 0.05 ng/ml in culture media.

Original language	English
Pages (from-to)	382-386
Number of pages	5
Journal	Afinidad
Volume	60
Issue number	506
Publication status	Published - Jul 2003

Keywords

Aspergillus ochraceus
HPLC
MS
Mycotoxins
Ochratoxin A
SPE

Cite this

@article{c8132131dfae4c68964eaca23e136274,

title = "Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector",

abstract = "A new, fast and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of ochratoxin A (OTA) production by fungi in culture media. The sample preparation consists of one step solid-phase extraction using a polymeric sorbent. The HPLC analysis uses a narrow-bore reversed-phase C18 column and an isocratic mobile phase composed of acetonitrile + water (0.1% HCOOH) (40+60) and fluorescence detector. The identity of this toxin was confirmed using electrospray ionization mass spectrometric detection (ESI/MS) in the positive ionization mode. The method had a recovery of 89.7% and a precision of 4.7% (n=6) for a sample which contains 17.3 ng/ml of OTA in culture media. The performance of the polymeric sorbent tested is as good as other studies published in the analysis of OTA. The detection limit of the new method is 0.05 ng/ml in culture media.",

keywords = "Aspergillus ochraceus, HPLC, MS, Mycotoxins, Ochratoxin A, SPE",

author = "Meritxell Ventura and Montserrat Agut and Francesc Broto-Puig and Llu{\'i}s Comellas",

year = "2003",

month = jul,

language = "English",

volume = "60",

pages = "382--386",

journal = "Afinidad",

issn = "0001-9704",

publisher = "Asociacion de Quimicos del Instituto Quimico de Sarria",

number = "506",

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TY - JOUR

T1 - Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector

AU - Ventura, Meritxell

AU - Agut, Montserrat

AU - Broto-Puig, Francesc

AU - Comellas, Lluís

PY - 2003/7

Y1 - 2003/7

N2 - A new, fast and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of ochratoxin A (OTA) production by fungi in culture media. The sample preparation consists of one step solid-phase extraction using a polymeric sorbent. The HPLC analysis uses a narrow-bore reversed-phase C18 column and an isocratic mobile phase composed of acetonitrile + water (0.1% HCOOH) (40+60) and fluorescence detector. The identity of this toxin was confirmed using electrospray ionization mass spectrometric detection (ESI/MS) in the positive ionization mode. The method had a recovery of 89.7% and a precision of 4.7% (n=6) for a sample which contains 17.3 ng/ml of OTA in culture media. The performance of the polymeric sorbent tested is as good as other studies published in the analysis of OTA. The detection limit of the new method is 0.05 ng/ml in culture media.

AB - A new, fast and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of ochratoxin A (OTA) production by fungi in culture media. The sample preparation consists of one step solid-phase extraction using a polymeric sorbent. The HPLC analysis uses a narrow-bore reversed-phase C18 column and an isocratic mobile phase composed of acetonitrile + water (0.1% HCOOH) (40+60) and fluorescence detector. The identity of this toxin was confirmed using electrospray ionization mass spectrometric detection (ESI/MS) in the positive ionization mode. The method had a recovery of 89.7% and a precision of 4.7% (n=6) for a sample which contains 17.3 ng/ml of OTA in culture media. The performance of the polymeric sorbent tested is as good as other studies published in the analysis of OTA. The detection limit of the new method is 0.05 ng/ml in culture media.

KW - Aspergillus ochraceus

KW - HPLC

KW - MS

KW - Mycotoxins

KW - Ochratoxin A

KW - SPE

UR - http://www.scopus.com/inward/record.url?scp=0345095432&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0345095432

SN - 0001-9704

VL - 60

SP - 382

EP - 386

JO - Afinidad

JF - Afinidad

IS - 506

ER -

Determination of ochratoxin A using solid-phase extraction and narrow-bore HPLC with fluorescence and ESI-MS detector

Abstract

Keywords

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