Design, synthesis and activity as acid ceramidase inhibitors of 2-oxooctanoyl and N-oleoylethanolamine analogues

Santiago Grijalvo, Carmen Bedia, Gemma Triola, Josefina Casas, Amadeu Llebaria, Jordi Teixidó, Obdulia Rabal, Thierry Levade, Antonio Delgado, Gemma Fabriàs

Research output: Indexed journal article Articlepeer-review

38 Citations (Scopus)

Abstract

The synthesis of novel N-acylethanolamines and their use as inhibitors of the aCDase is reported here. The compounds are either 2-oxooctanamides or oleamides of sphingosine analogs featuring a 3-hydroxy-4,5-hexadecenyl tail replaced by ether or thioether moieties. It appears that, within the 2-oxooctanamide family, the C3-OH group of the sphingosine molecule is required for inhibition both in vitro and in cultured cells. Furthermore, although the (E)-4 double bond is not essential for inhibitory activity, the (E) configuration is required, since the analogue with a (Z)-4 unsaturation was not inhibitory. None of the oleamides inhibited the aCDase in vitro. Conversely, with the exception of N-oleoylethanolamine and its analogs with S-decyl and S-hexadecyl substituents, all the synthesized oleamides inhibited the aCDase in cultured cells, although with a relatively low potency. We conclude that novel aCDase inhibitors can evolve from N-acylation of sphingoid bases with electron deficient-acyl groups. In contrast, chemical modification of the N-oleoylsphingosine backbone does not seem to offer an appropriate strategy to obtain aCDase inhibitors.

Original languageEnglish
Pages (from-to)69-84
Number of pages16
JournalChemistry and Physics of Lipids
Volume144
Issue number1
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Ceramidase
  • Ceramide
  • Farber disease
  • Inhibitor
  • Sphingolipids

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