Desarrollo de nuevos métodos para el análisis de doxiciclina en residuos orgánicos por HPLC

M. Gassiot; L. Comellas; F. Broto-Puig; D. Zandio; G. Mejía

Desarrollo de nuevos métodos para el análisis de doxiciclina en residuos orgánicos por HPLC

Translated title of the contribution: Development of new methods for the analysis of doxicycline in organic remainders by HPLC

M. Gassiot^*
, L. Comellas
, F. Broto-Puig
, D. Zandio
, G. Mejía

^*Corresponding author for this work

IQS School of Engineering

Research output: Indexed journal article › Article › peer-review

Abstract

Two simple fast methods have been developed to analyze doxicycline in surpluses of pigs by liquid chromatography. Sample preparation consists of a simple extraction with acidic water followed by a clean-up step carried out by solid phase extraction cartridges. Two inverse phase columns, a high speed column and a microbore, were evaluated for the chromatographic determination. A mobile phase of acetonitrile and water (0.1% HCOOH) was employed in all cases, gradient elution for the first column and an isocratic elution mode with the second. Detection was performed by mass spectroscopy with electrospray in positive mode. Recovery is over 70% and detection limits are 250 pg/Kg, when the microbore column is employed, and 500 pg/Kg, using the high speed one. By means of a MS-MS detector, detection limit is 10 μg/Kg.

Translated title of the contribution	Development of new methods for the analysis of doxicycline in organic remainders by HPLC
Original language	Spanish
Pages (from-to)	415-421
Number of pages	7
Journal	Afinidad
Volume	62
Issue number	519
Publication status	Published - Sept 2005

Cite this

@article{d3a93cacf5ab44f7a080d09748a6c64f,

title = "Desarrollo de nuevos m{\'e}todos para el an{\'a}lisis de doxiciclina en residuos org{\'a}nicos por HPLC",

abstract = "Two simple fast methods have been developed to analyze doxicycline in surpluses of pigs by liquid chromatography. Sample preparation consists of a simple extraction with acidic water followed by a clean-up step carried out by solid phase extraction cartridges. Two inverse phase columns, a high speed column and a microbore, were evaluated for the chromatographic determination. A mobile phase of acetonitrile and water (0.1\% HCOOH) was employed in all cases, gradient elution for the first column and an isocratic elution mode with the second. Detection was performed by mass spectroscopy with electrospray in positive mode. Recovery is over 70\% and detection limits are 250 pg/Kg, when the microbore column is employed, and 500 pg/Kg, using the high speed one. By means of a MS-MS detector, detection limit is 10 μg/Kg.",

keywords = "Doxicycline, HPLC, MS, Surpluses of pigs, Tetracycline",

author = "M. Gassiot and L. Comellas and F. Broto-Puig and D. Zandio and G. Mej{\'i}a",

year = "2005",

month = sep,

language = "Espa{\~n}ol",

volume = "62",

pages = "415--421",

journal = "Afinidad",

issn = "0001-9704",

publisher = "Asociacion de Quimicos del Instituto Quimico de Sarria",

number = "519",

}

TY - JOUR

T1 - Desarrollo de nuevos métodos para el análisis de doxiciclina en residuos orgánicos por HPLC

AU - Gassiot, M.

AU - Comellas, L.

AU - Broto-Puig, F.

AU - Zandio, D.

AU - Mejía, G.

PY - 2005/9

Y1 - 2005/9

N2 - Two simple fast methods have been developed to analyze doxicycline in surpluses of pigs by liquid chromatography. Sample preparation consists of a simple extraction with acidic water followed by a clean-up step carried out by solid phase extraction cartridges. Two inverse phase columns, a high speed column and a microbore, were evaluated for the chromatographic determination. A mobile phase of acetonitrile and water (0.1% HCOOH) was employed in all cases, gradient elution for the first column and an isocratic elution mode with the second. Detection was performed by mass spectroscopy with electrospray in positive mode. Recovery is over 70% and detection limits are 250 pg/Kg, when the microbore column is employed, and 500 pg/Kg, using the high speed one. By means of a MS-MS detector, detection limit is 10 μg/Kg.

AB - Two simple fast methods have been developed to analyze doxicycline in surpluses of pigs by liquid chromatography. Sample preparation consists of a simple extraction with acidic water followed by a clean-up step carried out by solid phase extraction cartridges. Two inverse phase columns, a high speed column and a microbore, were evaluated for the chromatographic determination. A mobile phase of acetonitrile and water (0.1% HCOOH) was employed in all cases, gradient elution for the first column and an isocratic elution mode with the second. Detection was performed by mass spectroscopy with electrospray in positive mode. Recovery is over 70% and detection limits are 250 pg/Kg, when the microbore column is employed, and 500 pg/Kg, using the high speed one. By means of a MS-MS detector, detection limit is 10 μg/Kg.

KW - Doxicycline

KW - HPLC

KW - MS

KW - Surpluses of pigs

KW - Tetracycline

UR - https://www.scopus.com/pages/publications/33745036535

M3 - Artículo

AN - SCOPUS:33745036535

SN - 0001-9704

VL - 62

SP - 415

EP - 421

JO - Afinidad

JF - Afinidad

IS - 519

ER -

Desarrollo de nuevos métodos para el análisis de doxiciclina en residuos orgánicos por HPLC

Abstract

Other files and links

Fingerprint

Cite this