Computer-assisted optimization in HPLC

L. G. Sabaté; A. M. Díaz; X. M. Tomás; M. M. Gassiot

doi:10.1093/chromsci/21.10.439

Computer-assisted optimization in HPLC

L. G. Sabaté^*
, A. M. Díaz
, X. M. Tomás
, M. M. Gassiot

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

11 Citations (Scopus)

Abstract

A program in BASIC is proposed for ‘off-line’ optimization of experimental parameters in HPLC by the Simplex sequential method. The program is modular and permits adaptation of the chromatographic resolution definition to the user’s needs with a minimum of programming knowledge. The program is applied to improve separation of 2,4-dinitrophenylhydrazones of isolated carbonyl compounds in beer. The optimized parameters are: flow, initial methanol concentration, concentration gradient, and initial isocratic time.

Original language	English
Pages (from-to)	439-443
Number of pages	5
Journal	Journal of Chromatographic Science
Volume	21
Issue number	10
DOIs	https://doi.org/10.1093/chromsci/21.10.439
Publication status	Published - Oct 1983
Externally published	Yes

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10.1093/chromsci/21.10.439

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AU - Tomás, X. M.

AU - Gassiot, M. M.

PY - 1983/10

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N2 - A program in BASIC is proposed for ‘off-line’ optimization of experimental parameters in HPLC by the Simplex sequential method. The program is modular and permits adaptation of the chromatographic resolution definition to the user’s needs with a minimum of programming knowledge. The program is applied to improve separation of 2,4-dinitrophenylhydrazones of isolated carbonyl compounds in beer. The optimized parameters are: flow, initial methanol concentration, concentration gradient, and initial isocratic time.

AB - A program in BASIC is proposed for ‘off-line’ optimization of experimental parameters in HPLC by the Simplex sequential method. The program is modular and permits adaptation of the chromatographic resolution definition to the user’s needs with a minimum of programming knowledge. The program is applied to improve separation of 2,4-dinitrophenylhydrazones of isolated carbonyl compounds in beer. The optimized parameters are: flow, initial methanol concentration, concentration gradient, and initial isocratic time.

UR - https://www.scopus.com/pages/publications/0020840871

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ER -

Computer-assisted optimization in HPLC

Abstract

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