Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy

Roger Riera, Jana Tauler, Natàlia Feiner-Gracia, Salvador Borrós, Cristina Fornaguera, Lorenzo Albertazzi

Research output: Indexed journal article Articlepeer-review

12 Citations (Scopus)

Abstract

Nanomedicine emerged some decades ago with the hope to be the solution for most unmet medical needs. However, tracking materials at nanoscale is challenging to their reduced size, below the resolution limit of most conventional techniques. In this context, we propose the use of direct stochastic optical reconstruction microscopy (dSTORM) to study time stability and cell trafficking after transfection of oligopeptide end-modified poly(β-aminoester) (OM-pBAE) nanoparticles. We selected different combinations of cationic end oligopeptides (arginine – R; histidine – H; and lysine – K) among polymer libraries, since the oligopeptide combination demonstrated to be useful for different applications, such as vaccination and gene silencing. We demonstrate that their time evolution as well as their cell uptake and trafficking are dependent on the oligopeptide. This study opens the pave to broad mechanistic studies at nanoscale that could enable a rational selection of specific pBAE nanoparticles composition after determining their stability and cell trafficking.

Original languageEnglish
Article numbere202100633
JournalChemMedChem
Volume17
Issue number13
DOIs
Publication statusPublished - 5 Jul 2022

Keywords

  • cell trafficking
  • direct stochastic optical reconstruction microscopy (dSTORM)
  • nanoparticle stability
  • poly(β-aminoester) nanoparticles

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