Changes in the physicochemical properties of transferrin upon chemical derivatization with ceramides

M. A. Egea*, M. L. García, M. A. Alsina, C. Mestres, F. Reig

*Corresponding author for this work

Research output: Indexed journal article Articlepeer-review

1 Citation (Scopus)

Abstract

The interaction of native and derivatized transferrin with lecithin, cholesterol and their mixtures was studied using monomolecular layers of phospholipids. Moreover, the thermodynamic parameters corresponding to these interactions: the mixing excess free energy, interaction energy, and interaction parameter were also calculated. The ability of these two proteins to insert into monolayers was studied working at constant area. The formation of aggregates or secondary structures in solution was determined by the fluorescence and polarization values of ANS (anilinonaphthalene sulfonate), measured at different protein/ANS relationships.

Original languageEnglish
Pages (from-to)570-576
Number of pages7
JournalColloid and Polymer Science
Volume272
Issue number5
DOIs
Publication statusPublished - May 1994
Externally publishedYes

Keywords

  • Ceramides
  • compression isotherms
  • liposomes
  • surface activity
  • transferrin

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