Biosynthesis of cyclic β-(1,2)-glucans in Rhizobium leguminosarum biovars viciae, phaseoli and trifolii

Olga A. Castro, Angeles Zorreguieta, Carlos Semino, Luis Ielpi

Research output: Indexed journal article Articlepeer-review

2 Citations (Scopus)

Abstract

Inner membranes of Rhizobium leguminosarum biovars viciae, phaseoli, and trifolii synthesized a heterogenous family of neutral cyclic β-(1,2)-glucans in a reaction system that used oligosaccharide intermediates covalently bound to a large protein. This glucoprotein showed a slightly lower mobility on SDS-polyacrylamide gels (apparent mol. mass of 320 kDa) than the β-(1,2)-glucan intermediate protein of Rhizobium meliloti. In vivo pulse-label experiments with growing cells of R. leguminosarum biovar trifolii RS800 using radioactive glucose showed that few species of cyclic β-(1,2)-glucans were synthesized and up to 30% were substituted with charged non-glycosidic residues, probably sn-1-phosphoglycerol.

Original languageEnglish
Pages (from-to)454-462
Number of pages9
JournalArchives of Microbiology
Volume163
Issue number6
DOIs
Publication statusPublished - Jun 1995
Externally publishedYes

Keywords

  • Biovar phaseoli
  • Biovar trifolii
  • Biovar viciae
  • Cyclic β-(1,2)-glucan
  • Glucoprotein intermediate
  • Polysaccharide synthesis
  • Rhizobium leguminosarum biovars

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