Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido; Aitziber L. Cortajarena; Joaquim Torra; Rubén Ruiz-González; Santi Nonell; Cristina Flors

doi:10.1039/c6cc03119f

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido
, Aitziber L. Cortajarena
, Joaquim Torra
, Rubén Ruiz-González
, Santi Nonell
, Cristina Flors^*

^*Corresponding author for this work

IQS School of Engineering

Research output: Indexed journal article › Article › peer-review

33 Citations (Scopus)

Abstract

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

Original language	English
Pages (from-to)	8405-8408
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	54
DOIs	https://doi.org/10.1039/c6cc03119f
Publication status	Published - 11 Jul 2016

Keywords

Singlet oxygen photosensitization
Fluorescent protein ilov
Electron-microscopy
Light
Photooxidation
Cytochemistry
Photophysics
Technology
Generation
Probes

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10.1039/c6cc03119f

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abstract = "Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.",

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AU - Rodríguez-Pulido, Alberto

AU - Cortajarena, Aitziber L.

AU - Torra, Joaquim

AU - Ruiz-González, Rubén

AU - Nonell, Santi

AU - Flors, Cristina

PY - 2016/7/11

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N2 - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

AB - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

KW - Singlet oxygen photosensitization

KW - Fluorescent protein ilov

KW - Electron-microscopy

KW - Light

KW - Photooxidation

KW - Cytochemistry

KW - Photophysics

KW - Technology

KW - Generation

KW - Probes

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Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Abstract

Keywords

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