Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

Roger Bresolí-Obach; Jaume Nos; Margarita Mora; Maria Lluïsa Sagristà; Rubén Ruiz-González; Santi Nonell

doi:10.1016/j.ymeth.2016.06.007

Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

Roger Bresolí-Obach
, Jaume Nos
, Margarita Mora
, Maria Lluïsa Sagristà
, Rubén Ruiz-González
, Santi Nonell^*

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

28 Citations (Scopus)

Abstract

We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.

Original language	English
Pages (from-to)	64-72
Number of pages	9
Journal	Methods
Volume	109
DOIs	https://doi.org/10.1016/j.ymeth.2016.06.007
Publication status	Published - 15 Oct 2016

Keywords

Anthracene dipropionic acid
Confocal microscopy
Fluorescent probe
HeLa cells
Silica nanoparticles
Singlet oxygen

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10.1016/j.ymeth.2016.06.007

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title = "Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media",

abstract = "We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.",

keywords = "Anthracene dipropionic acid, Confocal microscopy, Fluorescent probe, HeLa cells, Silica nanoparticles, Singlet oxygen",

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AU - Bresolí-Obach, Roger

AU - Nos, Jaume

AU - Mora, Margarita

AU - Sagristà, Maria Lluïsa

AU - Ruiz-González, Rubén

AU - Nonell, Santi

PY - 2016/10/15

Y1 - 2016/10/15

N2 - We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.

AB - We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.

KW - Anthracene dipropionic acid

KW - Confocal microscopy

KW - Fluorescent probe

KW - HeLa cells

KW - Silica nanoparticles

KW - Singlet oxygen

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EP - 72

JO - Methods

JF - Methods

ER -

Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

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Keywords

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