Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

Roger Bresolí-Obach; Jaume Nos; Margarita Mora; Maria Lluïsa Sagristà; Rubén Ruiz-González; Santi Nonell

doi:10.1016/j.ymeth.2016.06.007

Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

Roger Bresolí-Obach, Jaume Nos, Margarita Mora, Maria Lluïsa Sagristà, Rubén Ruiz-González, Santi Nonell

Research output: Indexed journal article › Article › peer-review

26 Citations (Scopus)

Abstract

We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.

Original language	English
Pages (from-to)	64-72
Number of pages	9
Journal	Methods
Volume	109
DOIs	https://doi.org/10.1016/j.ymeth.2016.06.007
Publication status	Published - 15 Oct 2016

Keywords

Anthracene dipropionic acid
Confocal microscopy
Fluorescent probe
HeLa cells
Silica nanoparticles
Singlet oxygen

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10.1016/j.ymeth.2016.06.007

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title = "Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media",

abstract = "We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.",

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AU - Bresolí-Obach, Roger

AU - Nos, Jaume

AU - Mora, Margarita

AU - Sagristà, Maria Lluïsa

AU - Ruiz-González, Rubén

AU - Nonell, Santi

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AB - We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.

KW - Anthracene dipropionic acid

KW - Confocal microscopy

KW - Fluorescent probe

KW - HeLa cells

KW - Silica nanoparticles

KW - Singlet oxygen

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Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media

Abstract

Keywords

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