Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection

J. Díaz; J. Ll Lliberia; L. Comellas; F. Broto-Puig

doi:10.1016/0021-9673(95)00372-X

Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection

J. Díaz
, J. Ll Lliberia
, L. Comellas^*
, F. Broto-Puig

^*Corresponding author for this work

IQS School of Engineering

Research output: Indexed journal article › Article › peer-review

66 Citations (Scopus)

Abstract

A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.

Original language	English
Pages (from-to)	171-179
Number of pages	9
Journal	Journal of Chromatography A
Volume	719
Issue number	1
DOIs	https://doi.org/10.1016/0021-9673(95)00372-X
Publication status	Published - 5 Jan 1996
Event	23rd Scientific Meeting of the Group-of-Chromatography-and-Related-Techniques of the Spanish-Royal-Society-of-Chemistry - PENISCOLA Duration: 19 Oct 1994 → 21 Oct 1994

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title = "Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection",

abstract = "A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.",

author = "J. D{\'i}az and Lliberia, \{J. Ll\} and L. Comellas and F. Broto-Puig",

note = "Funding Information: The authors thank the CICYT (Spain) (Reference: NAT 91-0340), for financial support and J.LI.L1 gratefully acknowledges the support of DGICYT (Spain).; 23rd Scientific Meeting of the Group-of-Chromatography-and-Related-Techniques of the Spanish-Royal-Society-of-Chemistry ; Conference date: 19-10-1994 Through 21-10-1994",

year = "1996",

month = jan,

day = "5",

doi = "10.1016/0021-9673(95)00372-X",

language = "English",

volume = "719",

pages = "171--179",

journal = "Journal of Chromatography A",

issn = "0021-9673",

publisher = "Elsevier B.V.",

number = "1",

}

Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection. / Díaz, J.; Lliberia, J. Ll; Comellas, L. et al.
In: Journal of Chromatography A, Vol. 719, No. 1, 05.01.1996, p. 171-179.

Research output: Indexed journal article › Article › peer-review

TY - JOUR

T1 - Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection

AU - Díaz, J.

AU - Lliberia, J. Ll

AU - Comellas, L.

AU - Broto-Puig, F.

N1 - Funding Information: The authors thank the CICYT (Spain) (Reference: NAT 91-0340), for financial support and J.LI.L1 gratefully acknowledges the support of DGICYT (Spain).

PY - 1996/1/5

Y1 - 1996/1/5

N2 - A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.

AB - A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.

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T2 - 23rd Scientific Meeting of the Group-of-Chromatography-and-Related-Techniques of the Spanish-Royal-Society-of-Chemistry

Y2 - 19 October 1994 through 21 October 1994

ER -

Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection

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