Fotosensibilitzadors codificables genèticament per a teràpia fotodinàmica

Project: Internal grants/own callsProjects

Project Details

Description

"The main goal of the project is to provide scientific basis for the implementation of personalized medicine in cutaneous melanoma. A new therapeutic strategy for orphan melanoma subtypes using photodynamic therapy will be explored for those patients which tumours are not carring mutations in the MAPkinase pathway.Within the proposed project we intend to develop, optimize and apply genetically encoded flavin-binding fluorescent proteins as light-controllable nano-generators for singlet oxygen (1O2). The release of this highly reactive oxygen species upon illumination allows to trigger complex systemic biological functions with high spatio-temporal resolution. To successfully integrate light-switchable 1O2 generators into functional macroscopic systems, we will address aspects of the entire research chain including:
1. The isolation, characterization and further improvement of flavin-binding fluorescent proteins as nanoscopic components capable of producing singlet oxygen with high efficiency.
2. The selective expression, localization and light-mediated activation of photosensitizing flavoproteins in different subcellular compartments, cell types (i.e. pathogenic bacterial and cancer cells) and macroscopic systems such as bacterial biofilms and tumors.
3. The demonstration of the biomedical applicability of flavoprotein-based photo-sensitizers for photo-dynamic inactivation of bacteria and cancer cells.
This project will be achieved through the close collaboration and synergism of clinicians, pathologists, basic scientists and
bioinformatics fully dedicated to cutaneous melanoma at different Institutions both in Catalonia and abroad."

Layman's description

"The main goal of the project is to provide scientific basis for the implementation of personalized medicine in cutaneous melanoma. A new therapeutic strategy for orphan melanoma subtypes using photodynamic therapy will be explored for those patients which tumours are not carring mutations in the MAPkinase pathway.Within the proposed project we intend to develop, optimize and apply genetically encoded flavin-binding fluorescent proteins as light-controllable nano-generators for singlet oxygen (1O2). The release of this highly reactive oxygen species upon illumination allows to trigger complex systemic biological functions with high spatio-temporal resolution. To successfully integrate light-switchable 1O2 generators into functional macroscopic systems, we will address aspects of the entire research chain including:
1. The isolation, characterization and further improvement of flavin-binding fluorescent proteins as nanoscopic components capable of producing singlet oxygen with high efficiency.
2. The selective expression, localization and light-mediated activation of photosensitizing flavoproteins in different subcellular compartments, cell types (i.e. pathogenic bacterial and cancer cells) and macroscopic systems such as bacterial biofilms and tumors.
3. The demonstration of the biomedical applicability of flavoprotein-based photo-sensitizers for photo-dynamic inactivation of bacteria and cancer cells.
This project will be achieved through the close collaboration and synergism of clinicians, pathologists, basic scientists and
bioinformatics fully dedicated to cutaneous melanoma at different Institutions both in Catalonia and abroad."
StatusFinished
Effective start/end date1/01/1531/12/15