Research output per year
Research output per year
- 44 results
Search results
-
2024
Enhanced quantification and cell tracking of dual fluorescent labeled extracellular vesicles
José Sánchez, M., Leivar, P., Borrós, S., Fornaguera, C. & Lecina, M., 25 Dec 2024, In: International Journal of Pharmaceutics. 667, Part A, 14 p., 124921.Research output: Indexed journal article › Article › peer-review
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Identification of critical process parameters for expansion of clinical grade human Wharton's jelly-derived mesenchymal stromal cells in stirred-tank bioreactors
López-Fernández, A., Lecina, M., Garcia Gragera, V. & Vives, J., Feb 2024, In: Biotechnology Journal. 19, 2, 14 p., 2300381.Research output: Indexed journal article › Article › peer-review
Open Access -
2022
Bioprocess characterization of virus-like particle production with the insect cell baculovirus expression system at nanoparticle level
Puente-Massaguer, E., González-Domínguez, I., Strobl, F., Grabherr, R., Striedner, G., Lecina, M. & Gòdia, F., Sept 2022, In: Journal of Chemical Technology and Biotechnology. 97, 9, p. 2456-2465 10 p.Research output: Indexed journal article › Article › peer-review
Open Access1 Citation (Scopus) -
Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use
Guarro, M., Suñer, F., Lecina, M., Borrós, S. & Fornaguera, C., Oct 2022, In: Colloids and Surfaces B: Biointerfaces. 218, 112745.Research output: Indexed journal article › Article › peer-review
14 Citations (Scopus) -
Monitoring Tools for the Development of High Cell Density Culture Strategies
Lecina Veciana, M., Comas, P., Martínez-Monge, I. & Cairó, J. J., 1 Feb 2022, Cell Culture Engineering and Technology. Springer Nature, p. 485-510 25 p.Research output: Book chapter › Chapter › peer-review
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2021
Accelerating HIV-1 VLP production using stable High Five insect cell pools
Puente-Massaguer, E., Grau-Garcia, P., Strobl, F., Grabherr, R., Striedner, G., Lecina, M. & Gòdia, F., Apr 2021, In: Biotechnology Journal. 16, 4, 2000391.Research output: Indexed journal article › Article › peer-review
14 Citations (Scopus) -
Stable Sf9 cell pools as a system for rapid HIV-1 virus-like particle production
Puente-Massaguer, E., Grau-Garcia, P., Strobl, F., Grabherr, R., Striedner, G., Lecina, M. & Gòdia, F., Dec 2021, In: Journal of Chemical Technology and Biotechnology. 96, 12, p. 3388-3397 10 p.Research output: Indexed journal article › Article › peer-review
4 Citations (Scopus) -
The effect of the expression of the antiapoptotic bhrf1 gene on the metabolic behavior of a hybridoma cell line
Martínez-Monge, I., Comas, P., Catalán-Tatjer, D., Prat, J., Casablancas, A., Paredes, C., Lecina, M. & Cairó, J. J., 2 Jul 2021, In: Applied Sciences (Switzerland). 11, 14, 6258.Research output: Indexed journal article › Article › peer-review
Open Access1 Citation (Scopus) -
2020
Application of advanced quantification techniques in nanoparticle-based vaccine development with the Sf9 cell baculovirus expression system
Puente-Massaguer, E., Lecina, M. & Gòdia, F., 11 Feb 2020, In: Vaccine. 38, 7, p. 1849-1859 11 p.Research output: Indexed journal article › Article › peer-review
18 Citations (Scopus) -
Coupling Microscopy and Flow Cytometry for a Comprehensive Characterization of Nanoparticle Production in Insect Cells
Puente-Massaguer, E., Saccardo, P., Ferrer-Miralles, N., Lecina, M. & Gòdia, F., 1 Sept 2020, In: Cytometry Part A. 97, 9, p. 921-932 12 p.Research output: Indexed journal article › Article › peer-review
8 Citations (Scopus) -
Development of a non-viral platform for rapid virus-like particle production in Sf9 cells
Puente-Massaguer, E., Gòdia, F. & Lecina, M., 10 Oct 2020, In: Journal of Biotechnology. 322, p. 43-53 11 p.Research output: Indexed journal article › Article › peer-review
17 Citations (Scopus) -
HEK293 cell-based bioprocess development at bench scale by means of online monitoring in shake flasks (RAMOS and SFR)
Anderlei, T., Keebler, M. V., Cairó, J. J. & Lecina, M., 2020, Methods in Molecular Biology. Humana Press Inc., p. 83-103 21 p. (Methods in Molecular Biology; vol. 2095).Research output: Book chapter › Chapter › peer-review
4 Citations (Scopus) -
Integrating nanoparticle quantification and statistical design of experiments for efficient HIV-1 virus-like particle production in High Five cells
Puente-Massaguer, E., Lecina, M. & Gòdia, F., 1 Feb 2020, In: Applied Microbiology and Biotechnology. 104, 4, p. 1569-1582 14 p.Research output: Indexed journal article › Article › peer-review
Open Access17 Citations (Scopus) -
PEI-mediated transient transfection of high five cells at bioreactor scale for HIV-1 VLP production
Puente-Massaguer, E., Strobl, F., Grabherr, R., Striedner, G., Lecina, M. & Gòdia, F., Aug 2020, In: Nanomaterials. 10, 8, p. 1-16 16 p., 1580.Research output: Indexed journal article › Article › peer-review
Open Access14 Citations (Scopus) -
Strategies for large-scale expansion of clinical-grade human multipotent mesenchymal stromal cells
García-Fernández, C., López-Fernández, A., Borrós, S., Lecina, M. & Vives, J., 15 Jul 2020, In: Biochemical Engineering Journal. 159, 12 p., 107601.Research output: Indexed journal article › Review › peer-review
18 Citations (Scopus) -
2019
Concomitant consumption of glucose and lactate: A novel batch production process for CHO cells
Martínez-Monge, I., Comas, P., Triquell, J., Casablancas, A., Lecina, M., Paredes, C. J. & Cairó, J. J., 15 Nov 2019, In: Biochemical Engineering Journal. 151, 107358.Research output: Indexed journal article › Article › peer-review
12 Citations (Scopus) -
Enabling HEK293 cells for antibiotic-free media bioprocessing through CRISPR/Cas9 gene editing
Román, R., Miret, J., Roura, A., Casablancas, A., Lecina, M. & Cairó, J. J., 15 Nov 2019, In: Biochemical Engineering Journal. 151, 107299.Research output: Indexed journal article › Article › peer-review
6 Citations (Scopus) -
Metabolic flux balance analysis during lactate and glucose concomitant consumption in HEK293 cell cultures
Martínez-Monge, I., Albiol, J., Lecina, M., Liste-Calleja, L., Miret, J., Solà, C. & Cairó, J. J., Feb 2019, In: Biotechnology and Bioengineering. 116, 2, p. 388-404 17 p.Research output: Indexed journal article › Article › peer-review
24 Citations (Scopus) -
New developments in online OUR monitoring and its application to animal cell cultures
Martínez-Monge, I., Roman, R., Comas, P., Fontova, A., Lecina, M., Casablancas, A. & Cairó, J. J., 5 Sept 2019, In: Applied Microbiology and Biotechnology. 103, 17, p. 6903-6917 15 p.Research output: Indexed journal article › Review › peer-review
22 Citations (Scopus) -
2018
A new strategy for fed-batch process control of HEK293 cell cultures based on alkali buffer addition monitoring: comparison with O.U.R. dynamic method
Martínez-Monge, I., Comas, P., Triquell, J., Lecina, M., Casablancas, A. & Cairó, J. J., 1 Dec 2018, In: Applied Microbiology and Biotechnology. 102, 24, p. 10469-10483 15 p.Research output: Indexed journal article › Article › peer-review
16 Citations (Scopus) -
A simplified implementation of the stationary liquid mass balance method for on-line OUR monitoring in animal cell cultures
Fontova, A., Lecina, M., López-Repullo, J., Martínez-Monge, I., Comas, P., Bragós, R. & Cairó, J. J., Jun 2018, In: Journal of Chemical Technology and Biotechnology. 93, 6, p. 1757-1766 10 p.Research output: Indexed journal article › Article › peer-review
10 Citations (Scopus) -
Effect of continuous feeding of CO2 and pH in cell concentration and product titers in hIFNγ producing HEK293 cells: Induced metabolic shift for concomitant consumption of glucose and lactate
Román, R., Farràs, M., Camps, M., Martínez-Monge, I., Comas, P., Martínez-Espelt, M., Lecina, M., Casablancas, A. & Cairó, J. J., 10 Dec 2018, In: Journal of Biotechnology. 287, p. 68-73 6 p.Research output: Indexed journal article › Article › peer-review
8 Citations (Scopus) -
Nanoscale characterization coupled to multi-parametric optimization of Hi5 cell transient gene expression
Puente-Massaguer, E., Lecina, M. & Gòdia, F., 1 Dec 2018, In: Applied Microbiology and Biotechnology. 102, 24, p. 10495-10510 16 p.Research output: Indexed journal article › Article › peer-review
19 Citations (Scopus) -
2017
Structural changes of Arthrospira sp. after low energy sonication treatment for microalgae harvesting: Elucidating key parameters to detect the rupture of gas vesicles
Lecina, M., Sanchez, B., Solà, C., Prat, J., Roldán, M., Hernández, M., Bragós, R., Paredes, C. J. & Cairó, J. J., 1 Jan 2017, In: Bioresource Technology. 223, p. 98-104 7 p.Research output: Indexed journal article › Article › peer-review
8 Citations (Scopus) -
2016
Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide
Román, R., Miret, J., Scalia, F., Casablancas, A., Lecina, M. & Cairó, J. J., 10 Dec 2016, In: Journal of Biotechnology. 239, p. 57-60 4 p.Research output: Indexed journal article › Article › peer-review
31 Citations (Scopus) -
Optimization of ferric chloride concentration and pH to improve both cell growth and flocculation in Chlorella vulgaris cultures. Application to medium reuse in an integrated continuous culture bioprocess
Lecina, M., Nadal, G., Solà, C., Prat, J. & Cairó, J. J., 1 Sept 2016, In: Bioresource Technology. 216, p. 211-218 8 p.Research output: Indexed journal article › Article › peer-review
13 Citations (Scopus) -
Real-time and on-line monitoring of morphological cell parameters using electrical impedance spectroscopy measurements
Sarró, E., Lecina, M., Fontova, A., Gòdia, F., Bragós, R. & Cairó, J. J., 1 Jun 2016, In: Journal of Chemical Technology and Biotechnology. 91, 6, p. 1755-1762 8 p.Research output: Indexed journal article › Article › peer-review
17 Citations (Scopus) -
2015
Lactate and glucose concomitant consumption as a self-regulated pH detoxification mechanism in HEK293 cell cultures
Liste-Calleja, L., Lecina, M., Lopez-Repullo, J., Albiol, J., Solà, C. & Cairó, J. J., 1 Dec 2015, In: Applied Microbiology and Biotechnology. 99, 23, p. 9951-9960 10 p.Research output: Indexed journal article › Article › peer-review
47 Citations (Scopus) -
Non-disruptive sonication of A. fusiformis (A. platensis) cultures facilitates its harvesting
Lecina, M., Prat, J., Paredes, C. J. & Cairó, J. J., 1 Jan 2015, In: Algal Research. 7, p. 1-4 4 p.Research output: Indexed journal article › Article › peer-review
6 Citations (Scopus) -
2014
HEK293 cell culture media study towards bioprocess optimization: Animal derived component free and animal derived component containing platforms
Liste-Calleja, L., Lecina, M. & Cairó, J. J., Apr 2014, In: Journal of Bioscience and Bioengineering. 117, 4, p. 471-477 7 p.Research output: Indexed journal article › Article › peer-review
21 Citations (Scopus) -
2013
Comparison of control strategies for fed-batch culture of hybridoma cells based on on-line monitoring of oxygen uptake rate, optical cell density and glucose concentration
Casablancas, A., Gámez, X., Lecina, M., Solà, C., Cairó, J. J. & Gòdia, F., Sept 2013, In: Journal of Chemical Technology and Biotechnology. 88, 9, p. 1680-1689 10 p.Research output: Indexed journal article › Article › peer-review
18 Citations (Scopus) -
IPTG limitation avoids metabolic burden and acetic acid accumulation in induced fed-batch cultures of Escherichia coli M15 under glucose limiting conditions
Lecina, M., Sarró, E., Casablancas, A., Gòdia, F. & Cairó, J. J., 15 Jan 2013, In: Biochemical Engineering Journal. 70, p. 78-83 6 p.Research output: Indexed journal article › Article › peer-review
25 Citations (Scopus) -
2012
Development of a simple disposable six minibioreactor system for suspension mammalian cell culture
Soley, A., Fontova, A., Gálvez, J., Sarró, E., Lecina, M., Bragós, R., Cairó, J. J. & Gòdia, F., Apr 2012, In: Process Biochemistry. 47, 4, p. 597-605 9 p.Research output: Indexed journal article › Article › peer-review
6 Citations (Scopus) -
Differentiation of human embryonic stem cells to cardiomyocytes on microcarrier cultures
Ting, S., Lecina, M., Reuveny, S. & Oh, S., 1 May 2012, In: Current Protocols in Stem Cell Biology. 21, 1, 1D.7.Research output: Indexed journal article › Article › peer-review
10 Citations (Scopus) -
Electrical impedance spectroscopy measurements using a four-electrode configuration improve on-line monitoring of cell concentration in adherent animal cell cultures
Sarró, E., Lecina, M., Fontova, A., Solà, C., Gòdia, F., Cairó, J. J. & Bragós, R., 15 Jan 2012, In: Biosensors and Bioelectronics. 31, 1, p. 257-263 7 p.Research output: Indexed journal article › Article › peer-review
44 Citations (Scopus) -
Optimization of HEK-293S cell cultures for the production of adenoviral vectors in bioreactors using on-line OUR measurements
Gálvez, J., Lecina, M., Solà, C., Cairó, J. J. & Gòdia, F., Jan 2012, In: Journal of Biotechnology. 157, 1, p. 214-222 9 p.Research output: Indexed journal article › Article › peer-review
29 Citations (Scopus) -
2011
Continuous perfusion culture of encapsulated hybridoma cells
Lecina, M., Tintó, A., Gálvez, J., Gòdia, F. & Cairó, J. J., Dec 2011, In: Journal of Chemical Technology and Biotechnology. 86, 12, p. 1555-1564 10 p.Research output: Indexed journal article › Article › peer-review
7 Citations (Scopus) -
Distinct regulation of mitogen-activated protein kinase activities is coupled with enhanced cardiac differentiation of human embryonic stem cells
Kempf, H., Lecina, M., Ting, S., Zweigerdt, R. & Oh, S., Nov 2011, In: Stem Cell Research. 7, 3, p. 198-209 12 p.Research output: Indexed journal article › Article › peer-review
Open Access25 Citations (Scopus) -
2010
Scalable platform for human embryonic stem cell differentiation to cardiomyocytes in suspended microcarrier cultures
Lecina, M., Ting, S., Choo, A., Reuveny, S. & Oh, S., 1 Dec 2010, In: Tissue Engineering - Part C: Methods. 16, 6, p. 1609-1619 11 p.Research output: Indexed journal article › Article › peer-review
99 Citations (Scopus) -
2008
Continuous oxygen consumption estimation method for animal cell bioreactors based on a low-cost control of the medium dissolved oxygen concentration
Fontova, A., Soley, A., Gálvez, J., Sarró, E., Lecina, M., Rosell, J., Riu, P. J., Cairó, J., Gòdia, F. & Bragos, R., 2008, 4th European Conference of the International Federation for Medical and Biological Engineering - ECIFMBE 2008. p. 2190-2194 5 p. (IFMBE Proceedings; vol. 22).Research output: Book chapter › Conference contribution › peer-review
1 Citation (Scopus) -
Microcarriers for stem cell culture
Oh, S. (Inventor), Lecina Veciana, M. (Inventor), Choo, A. (Inventor), Reuveny, S. (Inventor), Zweigerdt, R. (Inventor) & Chen, A. (Inventor), 17 Mar 2008, Patent No. US 14/134,275, Priority date 17 Mar 2008Research output: Patent › PCT Application
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2006
Application of on-line OUR measurements to detect actions points to improve baculovirus-insect cell cultures in bioreactors
Lecina, M., Soley, A., Gràcia, J., Espunya, E., Lázaro, B., Cairó, J. J. & Gòdia, F., 18 Sept 2006, In: Journal of Biotechnology. 125, 3, p. 385-394 10 p.Research output: Indexed journal article › Article › peer-review
24 Citations (Scopus) -
Multiple automated minibioreactor system for multifunctional screening in biotechnology
Fontova, A., Soley, A., Gálvez, J., Sarró, E., Lecina, M., Rosell, J., Riu, P., Cairó, J., Gòdia, F. & Bragós, R., 2006, 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06. p. 632-635 4 p. 4030412. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).Research output: Book chapter › Conference contribution › peer-review
5 Citations (Scopus) -
2005
On-line monitoring of yeast cell growth by impedance spectroscopy
Soley, A., Lecina, M., Gámez, X., Cairó, J. J., Riu, P., Rosell, X., Bragós, R. & Gòdia, F., 10 Sept 2005, In: Journal of Biotechnology. 118, 4, p. 398-405 8 p.Research output: Indexed journal article › Article › peer-review
69 Citations (Scopus)