TY - JOUR
T1 - Zinc-Substituted Myoglobin Is a Naturally Occurring Photo-antimicrobial Agent with Potential Applications in Food Decontamination
AU - Delcanale, Pietro
AU - Montali, Chiara
AU - Rodríguez-Amigo, Beatriz
AU - Abbruzzetti, Stefania
AU - Bruno, Stefano
AU - Bianchini, Paolo
AU - Diaspro, Alberto
AU - Agut, Montserrat
AU - Nonell, Santi
AU - Viappiani, Cristiano
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/11/16
Y1 - 2016/11/16
N2 - Zinc-substituted myoglobin (ZnMb) is a naturally occurring photosensitizer that generates singlet oxygen with a high quantum yield. Using a combination of photophysical and fluorescence imaging techniques, we demonstrate the interaction of ZnMb with Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. An efficient antibacterial action against S. aureus was observed, with a reduction up to 99.9999% in the number of colony-forming units, whereas no sizable effect was detected against E. coli. Because ZnMb is known to form during the maturation of additive-free not-cooked cured ham, the use of this protein as a built-in photodynamic agent may constitute a viable method for the decontamination of these food products from Gram-positive bacteria.
AB - Zinc-substituted myoglobin (ZnMb) is a naturally occurring photosensitizer that generates singlet oxygen with a high quantum yield. Using a combination of photophysical and fluorescence imaging techniques, we demonstrate the interaction of ZnMb with Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. An efficient antibacterial action against S. aureus was observed, with a reduction up to 99.9999% in the number of colony-forming units, whereas no sizable effect was detected against E. coli. Because ZnMb is known to form during the maturation of additive-free not-cooked cured ham, the use of this protein as a built-in photodynamic agent may constitute a viable method for the decontamination of these food products from Gram-positive bacteria.
KW - antibacterial photosensitization-based treatment
KW - natural photosensitizers
KW - singlet oxygen
UR - http://www.scopus.com/inward/record.url?scp=84996600323&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.6b03368
DO - 10.1021/acs.jafc.6b03368
M3 - Article
C2 - 27785913
AN - SCOPUS:84996600323
SN - 0021-8561
VL - 64
SP - 8633
EP - 8639
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 45
ER -