Versatile Supramolecular Complex for Targeted Antimicrobial Photodynamic Inactivation

Andrea Mussini, Eleonora Uriati, Cormac Hally, Santi Nonell, Paolo Bianchini, Alberto Diaspro, Stefano Pongolini, Pietro Delcanale, Stefania Abbruzzetti, Cristiano Viappiani

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4 Cites (Scopus)

Resum

We report the development of a supramolecular structure endowed with photosensitizing properties and targeting capability for antimicrobial photodynamic inactivation. Our synthetic strategy uses the tetrameric bacterial protein streptavidin, labeled with the photosensitizer eosin, as the main building block. Biotinylated immunoglobulin G (IgG) from human serum, known to associate with Staphylococcus aureus protein A, was bound to the complex streptavidin-eosin. Fluorescence correlation spectroscopy and fluorescence microscopy demonstrate binding of the complex to S. aureus. Efficient photoinactivation is observed for S. aureus suspensions treated with IgG-streptavidin-eosin at concentrations higher than 0.5 μM and exposed to green light. The proposed strategy offers a flexible platform for targeting a variety of molecules and microbial species.

Idioma originalAnglès
Pàgines (de-a)666-676
Nombre de pàgines11
RevistaBioconjugate Chemistry
Volum33
Número4
DOIs
Estat de la publicacióPublicada - 20 d’abr. 2022

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