Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production

M Lecina; A Casablancas; A Soley; C Vela; E Espuña; J De Gracia; JJ Cairó; F Gódia

Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production

M Lecina, A Casablancas, A Soley, C Vela, E Espuña, J De Gracia, JJ Cairó, F Gódia

Producció científica: Capítol de llibre › Contribució a congrés/conferència › Avaluat per experts

Resum

On-line oxygen uptake rate measurements using a dynamic model are applied to Sf9 cultures infected with recombinant baculovirus. This measurement allows a sensitive monitoring of cell growth and post-infection process. Also, results are given on fed-batch and perfusion cultures were 8-10 (.) 10(6) cells/ml can be achieved by automatically adjusting the medium fed-rate on the basis of OUR on-line measurements.

Idioma original	Anglès
Títol de la publicació	Animal Cell Technology Meets Genomics
Editors	F Godia, M Fussenegger
Editor	Springer Nature
Pàgines	779-781
Nombre de pàgines	3
ISBN (imprès)	1-4020-2791-5
Estat de la publicació	Publicada - 2005
Publicat externament	Sí
Esdeveniment	18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) - Granada, Spain Durada: 11 de maig 2003 → 14 de maig 2003

Conferència

Conferència	18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT)
País/Territori	Spain
Ciutat	Granada
Període	11/05/03 → 14/05/03

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@inproceedings{d8e445e5673a424481b02bf24b6e0cb1,

title = "Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production",

abstract = "On-line oxygen uptake rate measurements using a dynamic model are applied to Sf9 cultures infected with recombinant baculovirus. This measurement allows a sensitive monitoring of cell growth and post-infection process. Also, results are given on fed-batch and perfusion cultures were 8-10 (.) 10(6) cells/ml can be achieved by automatically adjusting the medium fed-rate on the basis of OUR on-line measurements.",

author = "M Lecina and A Casablancas and A Soley and C Vela and E Espu{\~n}a and \{De Gracia\}, J and JJ Cair{\'o} and F G{\'o}dia",

year = "2005",

language = "English",

isbn = "1-4020-2791-5",

pages = "779--781",

editor = "F Godia and M Fussenegger",

booktitle = "Animal Cell Technology Meets Genomics",

publisher = "Springer Nature",

address = "United Kingdom",

note = "18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) ; Conference date: 11-05-2003 Through 14-05-2003",

}

Lecina, M, Casablancas, A, Soley, A, Vela, C, Espuña, E, De Gracia, J, Cairó, JJ & Gódia, F 2005, Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production. in F Godia & M Fussenegger (ed.), Animal Cell Technology Meets Genomics. Springer Nature, pàg. 779-781, 18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT), Granada, Spain, 11/05/03.

Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production. / Lecina, M; Casablancas, A; Soley, A et al.
Animal Cell Technology Meets Genomics. ed. / F Godia; M Fussenegger. Springer Nature, 2005. pàg. 779-781.

Producció científica: Capítol de llibre › Contribució a congrés/conferència › Avaluat per experts

TY - GEN

T1 - Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production

AU - Lecina, M

AU - Casablancas, A

AU - Soley, A

AU - Vela, C

AU - Espuña, E

AU - De Gracia, J

AU - Cairó, JJ

AU - Gódia, F

PY - 2005

Y1 - 2005

N2 - On-line oxygen uptake rate measurements using a dynamic model are applied to Sf9 cultures infected with recombinant baculovirus. This measurement allows a sensitive monitoring of cell growth and post-infection process. Also, results are given on fed-batch and perfusion cultures were 8-10 (.) 10(6) cells/ml can be achieved by automatically adjusting the medium fed-rate on the basis of OUR on-line measurements.

AB - On-line oxygen uptake rate measurements using a dynamic model are applied to Sf9 cultures infected with recombinant baculovirus. This measurement allows a sensitive monitoring of cell growth and post-infection process. Also, results are given on fed-batch and perfusion cultures were 8-10 (.) 10(6) cells/ml can be achieved by automatically adjusting the medium fed-rate on the basis of OUR on-line measurements.

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M3 - Conference contribution

SN - 1-4020-2791-5

SP - 779

EP - 781

BT - Animal Cell Technology Meets Genomics

A2 - Godia, F

A2 - Fussenegger, M

PB - Springer Nature

T2 - 18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT)

Y2 - 11 May 2003 through 14 May 2003

ER -

Use of on-line our measurements to monitor and improve insect cell cultures for recombinant protein production

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