TY - JOUR
T1 - Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices
AU - Delpino-Rius, Antoni
AU - Eras, Jordi
AU - Marsol-Vall, Alexis
AU - Vilaró, Francisca
AU - Balcells, Mercè
AU - Canela-Garayoa, Ramon
PY - 2014/2/28
Y1 - 2014/2/28
N2 - We have developed an analytical method that allows the simultaneous determination of epoxycarotenoids, hydroxycarotenoids and carotenes in monovarietal fresh homemade and industrially processed fruit products. Analyses were carried out using ultra performance liquid chromatography (UPLC). The extraction method was optimized using methanol as the first extraction solvent for lyophilized samples followed by a saponification step. Recoveries ranged between 75% and 104% depending on the compound. Repeatability was better than 10% for all compounds (%RSD, n= 3). The chromatographic analysis takes less than 17. min. In this short period, up to 27 carotenoids were identified in apple, peach and pear products. The developed method allowed us to differentiate juice from six varieties of apple by their carotenoid profile. Moreover, the methodology allows us to differentiate the carotenoid profiles from commercial juices and homemade fresh peach and pear juices, as well as to study the rearrangements of 5,6- to 5,8-epoxycarotenoids.
AB - We have developed an analytical method that allows the simultaneous determination of epoxycarotenoids, hydroxycarotenoids and carotenes in monovarietal fresh homemade and industrially processed fruit products. Analyses were carried out using ultra performance liquid chromatography (UPLC). The extraction method was optimized using methanol as the first extraction solvent for lyophilized samples followed by a saponification step. Recoveries ranged between 75% and 104% depending on the compound. Repeatability was better than 10% for all compounds (%RSD, n= 3). The chromatographic analysis takes less than 17. min. In this short period, up to 27 carotenoids were identified in apple, peach and pear products. The developed method allowed us to differentiate juice from six varieties of apple by their carotenoid profile. Moreover, the methodology allows us to differentiate the carotenoid profiles from commercial juices and homemade fresh peach and pear juices, as well as to study the rearrangements of 5,6- to 5,8-epoxycarotenoids.
KW - Carotenoid profile
KW - Epoxycarotenoid
KW - Hydroxycarotenoid
KW - Monovarietal fruit juice
KW - UPLC
UR - http://www.scopus.com/inward/record.url?scp=84896721052&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2014.01.044
DO - 10.1016/j.chroma.2014.01.044
M3 - Article
C2 - 24503121
AN - SCOPUS:84896721052
SN - 0021-9673
VL - 1331
SP - 90
EP - 99
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -