TY - JOUR
T1 - Toxicogenetic monitoring in urban cities exposed to different airborne contaminants
AU - Pereira, Tatiana S.
AU - Beltrami, Laiana S.
AU - Rocha, Jocelita A.V.
AU - Broto, Francesc P.
AU - Comellas, Lluis R.
AU - Salvadori, Daisy M.F.
AU - Vargas, Vera M.F.
N1 - Funding Information:
The authors are grateful to all volunteers, to Secretaria Municipal de Saúde of Montenegro, to Secretaria Municipal de Saúde e Meio Ambiente of Santo Antônio da Patrulha and to FEPAM for providing the air sample filters needed to develop the study. We also thank Mark B. Yunker for suggestions and Mariana V. Coronas for her aid. This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant nos. 472673/2004-1 , 303424/2005-2 , 303151/2008-0 ) which also granted Scientific Initiation scholarships to Laiana Beltrami and a Further Training scholarship to Jocelita A.V. Rocha. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Grant no. 2891/06-9 ) granted doctoral fellowships (regular and Sandwich program) to Tatiana S. Pereira.
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Microparticles found in the air may be associated with organic matter that contains several compounds, such as Polycyclic Aromatic Hydrocarbons (PAHs) and nitro-PAHs, and may pose a significant risk to human health, possibly leading to DNA mutations and cancers. This study associated genotoxicity assays for evaluating human exposure with the atmospheric air of two urban areas in southern Brazil, that received different atmospheric contributions. Site 1 was under urban-industrial influence and the other was a non-industrial reference, Site 2. Organic extracts from the airborne particulate matter were tested for mutagenicity via the Salmonella/microsome assay and analyzed for PAH composition. Cells samples of people residing in these two cities were evaluated using the comet and micronucleus assay (MN).Concentrations of the individual PAHs ranged from 0.01ng/m3 (benzo[a]anthracene) to 5.08ng/m3 (benzo[ghi]perylene). As to mutagenicity analysis of airborne, Site 1 presented all the mutagenic responses, which varied from 3.2±1.22rev/m3 (TA98 no S9) to 32.6±2.05rev/m3 (TA98, S9), while Site 2 ranged from negative to minimal responses. Site 1 presented a high quantity of nitro and amino derivatives of PAHs, and peaked at 56.0±3.68rev/μg (YG1024 strain). The two groups presented very low DNA damage levels without intergroup difference. Although Site 1 presented high mutagenic responses in the air samples, high PAH levels, healthy people exposed to this environment did not show significative damage in their genetic material. However, the evaluation of different environmental and genetic damage in such population is necessary to monitor possible damages.
AB - Microparticles found in the air may be associated with organic matter that contains several compounds, such as Polycyclic Aromatic Hydrocarbons (PAHs) and nitro-PAHs, and may pose a significant risk to human health, possibly leading to DNA mutations and cancers. This study associated genotoxicity assays for evaluating human exposure with the atmospheric air of two urban areas in southern Brazil, that received different atmospheric contributions. Site 1 was under urban-industrial influence and the other was a non-industrial reference, Site 2. Organic extracts from the airborne particulate matter were tested for mutagenicity via the Salmonella/microsome assay and analyzed for PAH composition. Cells samples of people residing in these two cities were evaluated using the comet and micronucleus assay (MN).Concentrations of the individual PAHs ranged from 0.01ng/m3 (benzo[a]anthracene) to 5.08ng/m3 (benzo[ghi]perylene). As to mutagenicity analysis of airborne, Site 1 presented all the mutagenic responses, which varied from 3.2±1.22rev/m3 (TA98 no S9) to 32.6±2.05rev/m3 (TA98, S9), while Site 2 ranged from negative to minimal responses. Site 1 presented a high quantity of nitro and amino derivatives of PAHs, and peaked at 56.0±3.68rev/μg (YG1024 strain). The two groups presented very low DNA damage levels without intergroup difference. Although Site 1 presented high mutagenic responses in the air samples, high PAH levels, healthy people exposed to this environment did not show significative damage in their genetic material. However, the evaluation of different environmental and genetic damage in such population is necessary to monitor possible damages.
KW - Comet assay
KW - Human environmental exposure
KW - Mutagenicity
KW - Polycyclic aromatic hydrocarbon
KW - Salmonella/microsome assay
KW - Total airborne particulate matter
UR - http://www.scopus.com/inward/record.url?scp=84874280888&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2012.12.029
DO - 10.1016/j.ecoenv.2012.12.029
M3 - Article
C2 - 23395453
AN - SCOPUS:84874280888
SN - 0147-6513
VL - 90
SP - 174
EP - 182
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -
