TY - JOUR
T1 - The role of hydrophobic alkyl chains in the physicochemical properties of poly(β-amino ester)/DNA complexes
AU - Rata-Aguilar, Azahara
AU - Segovia-Ramos, Nathaly
AU - Jódar-Reyes, Ana Belén
AU - Ramos-Pérez, Víctor
AU - Borrós, Salvador
AU - Ortega-Vinuesa, Juan Luis
AU - Martín-Rodríguez, Antonio
N1 - Funding Information:
The authors wish to express their appreciation for the financial support granted by the following research projects: MAT2013-43922-R and MAT2010-20370 – European FEDER support included – (MECD, Spain), P07-FQM3099 and P10-CTS-6270 ( Junta de Andalucía, Spain ), CEIBiotic 20F12/16 . Azahara Rata-Aguilar thanks the Government of Spain (MECD) for her FPU fellowship. GEMAT group would like to thank Generalitat de Catalunya for its support through the Consolidated Grant 2009 SGR 1461
Publisher Copyright:
© 2015.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Two degradable poly(β-amino ester)s with an average molecular weight of 2. kDa, referred to as B1 and B2, have been synthesized to be tested as non-viral gene delivery systems. B2 polymer exhibits two additional non-polar ethyl groups at both ends. This paper describes the influence of that subtle difference on the compaction ability and temporal stability of the complexes formed with plasmid DNA. Our results suggest that the inclusion of those small hydrophobic fragments into the polycation backbone improves its suitability as synthetic DNA carrier. The improvement is related to the formation and physicochemical properties of the complexes. B2 polyplexes were more stable, the polymer hydrolysis was slowed down and plasmid DNA was better protected which was translated into better transfection efficiencies. Although still not totally understood, the role played by hydrophobic forces is ubiquitous in chemical, biological and physical systems, and they must be considered to design future polymers for gene delivery.
AB - Two degradable poly(β-amino ester)s with an average molecular weight of 2. kDa, referred to as B1 and B2, have been synthesized to be tested as non-viral gene delivery systems. B2 polymer exhibits two additional non-polar ethyl groups at both ends. This paper describes the influence of that subtle difference on the compaction ability and temporal stability of the complexes formed with plasmid DNA. Our results suggest that the inclusion of those small hydrophobic fragments into the polycation backbone improves its suitability as synthetic DNA carrier. The improvement is related to the formation and physicochemical properties of the complexes. B2 polyplexes were more stable, the polymer hydrolysis was slowed down and plasmid DNA was better protected which was translated into better transfection efficiencies. Although still not totally understood, the role played by hydrophobic forces is ubiquitous in chemical, biological and physical systems, and they must be considered to design future polymers for gene delivery.
KW - Biodegradable polymer
KW - Colloidal stability
KW - Hydrophobic interactions
KW - Nonviral DNA carriers
KW - Poly(β-amino ester)
KW - Polyplexes
UR - http://www.scopus.com/inward/record.url?scp=84923002858&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2014.11.048
DO - 10.1016/j.colsurfb.2014.11.048
M3 - Article
C2 - 25601090
AN - SCOPUS:84923002858
SN - 0927-7765
VL - 126
SP - 374
EP - 380
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -