TY - JOUR
T1 - Tetramethylbenzidine
T2 - An acoustogenic photoacoustic probe for reactive oxygen species detection
AU - Bresolí-Obach, Roger
AU - Frattini, Marcello
AU - Abbruzzetti, Stefania
AU - Viappiani, Cristiano
AU - Agut, Montserrat
AU - Nonell, Santi
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10/2
Y1 - 2020/10/2
N2 - Photoacoustic imaging is attracting a great deal of interest owing to its distinct advantages over other imaging techniques such as fluorescence or magnetic resonance image. The availability of photoacoustic probes for reactive oxygen and nitrogen species (ROS/RNS) could shed light on a plethora of biological processes mediated by these key intermediates. Tetramethylbenzidine (TMB) is a non-toxic and non-mutagenic colorless dye that develops a distinctive blue color upon oxidation. In this work, we have investigated the potential of TMB as an acoustogenic photoacoustic probe for ROS/RNS. Our results indicate that TMB reacts with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce the blue oxidation product, while ROS, such as the superoxide radical anion, sodium peroxide, hydroxyl radical, or peroxynitrite, yield a colorless oxidation product. TMB does not penetrate the Escherichia coli cytoplasm but is capable of detecting singlet oxygen generated in its outer membrane.
AB - Photoacoustic imaging is attracting a great deal of interest owing to its distinct advantages over other imaging techniques such as fluorescence or magnetic resonance image. The availability of photoacoustic probes for reactive oxygen and nitrogen species (ROS/RNS) could shed light on a plethora of biological processes mediated by these key intermediates. Tetramethylbenzidine (TMB) is a non-toxic and non-mutagenic colorless dye that develops a distinctive blue color upon oxidation. In this work, we have investigated the potential of TMB as an acoustogenic photoacoustic probe for ROS/RNS. Our results indicate that TMB reacts with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce the blue oxidation product, while ROS, such as the superoxide radical anion, sodium peroxide, hydroxyl radical, or peroxynitrite, yield a colorless oxidation product. TMB does not penetrate the Escherichia coli cytoplasm but is capable of detecting singlet oxygen generated in its outer membrane.
KW - 3,3,5,5-tetramethylbenzidine
KW - Optical sensors
KW - Photoacoustic probes
KW - Reactive nitrogen species (RNS)
KW - Reactive oxygen species (ROS)
KW - Singlet oxygen
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UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000585608400001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.3390/s20205952
DO - 10.3390/s20205952
M3 - Article
C2 - 33096750
AN - SCOPUS:85093873427
SN - 1424-8220
VL - 20
SP - 1
EP - 16
JO - Sensors (Switzerland)
JF - Sensors (Switzerland)
IS - 20
M1 - 5952
ER -