Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells

Jangho Kim, Hong Nam Kim, Ki Taek Lim, Yeonju Kim, Shambhavi Pandey, Pankaj Garg, Yun Hoon Choung, Pill Hoon Choung, Kahp Yang Suh, Jong Hoon Chung*

*Autor corresponent d’aquest treball

Producció científica: Article en revista indexadaArticleAvaluat per experts

100 Cites (Scopus)

Resum

Inspired by the aligned nanostructures and co-existence of vascular cells and stem cells in human cancellous bone, we quantitatively investigated the relative contributions of nanotopography and co-culture with human umbilical endothelial cells (HUVECs) to the osteogenesis of human mesenchymal stem cells (hMSCs). Although both nanotopography and co-culture independently enhanced the osteogenesis of hMSCs, osteogenesis was further enhanced by the two factors in combination, indicating the importance of synergistic cues in stem cell engineering. Interestingly, nanotopography provided a larger relative contribution to the osteogenesis of hMSCs than did co-culture with HUVECs. Furthermore, the osteogenesis of hMSCs was also affected by the density of parallel nanogrooves, exhibiting a maximum at a 1:3 spacing ratio, as defined as the ratio of ridge width to groove width. Analysis of (i) biochemical soluble factors, (ii) hMSC-substrate interaction and (iii) hMSC-HUVEC interaction suggests that (ii) and (iii) play a crucial role in mediating osteogenic phenotypes.

Idioma originalAnglès
Pàgines (de-a)7257-7268
Nombre de pàgines12
RevistaBiomaterials
Volum34
Número30
DOIs
Estat de la publicacióPublicada - d’oct. 2013
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