TY - JOUR
T1 - Structural changes of Arthrospira sp. after low energy sonication treatment for microalgae harvesting
T2 - Elucidating key parameters to detect the rupture of gas vesicles
AU - Lecina, Martí
AU - Sanchez, Benjamin
AU - Solà, Carles
AU - Prat, Jordi
AU - Roldán, Mònica
AU - Hernández, Mariona
AU - Bragós, Ramon
AU - Paredes, Carlos J.
AU - Cairó, Jordi J.
N1 - Funding Information:
The project was supported by the Ministerio de Economia y Competitividad of the Spanish Government (INNPACTO, IPT-2012-0249-310000).
Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The buoyancy suppression by low energy sonication (LES) treatment (0.8 W·mL−1, 20 kHz, 10 s) has recently been proposed as an initial harvesting step for Arthrospira sp. This paper aims to describe the structural changes in Arthrospira sp. after LES treatment and to present how these structural changes affect the results obtained by different analytical techniques. Transmission electron microscopy (TEM) micrographs of trichomes evidenced the gas vesicles rupture but also revealed a rearrangement of thylakoids and more visible phycobilisomes were observed. Differences between treated and untreated samples were detected by confocal microscopy, flow cytometry and optical microscopy but not by electrical impedance spectroscopy (EIS). After LES treatment, 2-fold increase in autofluorescence at 610/660 nm was measured (phycocyanin/allophycocyanin emission wavelengths) and a ten-fold decrease in side scatter light intensity (due to a reduction of trichome's inner complexity). This was further confirmed by optical microscopy showing changes on trichomes appearance (from wrinkled to smooth).
AB - The buoyancy suppression by low energy sonication (LES) treatment (0.8 W·mL−1, 20 kHz, 10 s) has recently been proposed as an initial harvesting step for Arthrospira sp. This paper aims to describe the structural changes in Arthrospira sp. after LES treatment and to present how these structural changes affect the results obtained by different analytical techniques. Transmission electron microscopy (TEM) micrographs of trichomes evidenced the gas vesicles rupture but also revealed a rearrangement of thylakoids and more visible phycobilisomes were observed. Differences between treated and untreated samples were detected by confocal microscopy, flow cytometry and optical microscopy but not by electrical impedance spectroscopy (EIS). After LES treatment, 2-fold increase in autofluorescence at 610/660 nm was measured (phycocyanin/allophycocyanin emission wavelengths) and a ten-fold decrease in side scatter light intensity (due to a reduction of trichome's inner complexity). This was further confirmed by optical microscopy showing changes on trichomes appearance (from wrinkled to smooth).
KW - Arthrospira sp.
KW - Gas vesicles rupture
KW - Low energy sonication treatment
KW - Microalgae harvesting monitoring
KW - Morphological/structural changes
UR - http://www.scopus.com/inward/record.url?scp=84992560076&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.10.030
DO - 10.1016/j.biortech.2016.10.030
M3 - Article
C2 - 27788433
AN - SCOPUS:84992560076
SN - 0960-8524
VL - 223
SP - 98
EP - 104
JO - Bioresource Technology
JF - Bioresource Technology
ER -
