Stereochemical course and structure of the products of the enzymic action of endo-1,3-1,4-β-D-glucan 4-glucanohydrolase from Bacillus licheniformis

C. Malet, J. Jimenez-Barbero, M. Bernabe, C. Brosa, A. Planas

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Resum

The stereochemical course of the reaction catalysed by endo-1,3-1,4-β-D-glucan 4-glucanohydrolase (EC 3.2.1.73) has been determined by 1H n.m.r. The enzyme-catalysed hydrolysis of barley β-glucan proceeds with overall retention of the anomeric configuration, indicating that the enzyme operates through a double-displacement mechanism. The structures of the final oligo-saccharide products, 3-β-O-cellobiosyl D-glucopyranoside and 3-β-O-cellotriosyl D-glucopyranoside, have been completely assigned by 1H- and 13C-n.m.r. spectroscopy.

Idioma originalAnglès
Pàgines (de-a)753-758
Nombre de pàgines6
RevistaBiochemical Journal
Volum296
Número3
DOIs
Estat de la publicacióPublicada - 1993

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