Singlet oxygen photosensitisation by GFP mutants: Oxygen accessibility to the chromophore

Ana Jiménez-Banzo, Xavier Ragàs, Stefania Abbruzzetti, Cristiano Viappiani, Barbara Campanini, Cristina Flors, Santi Nonell

Producció científica: Article en revista indexadaArticleAvaluat per experts

32 Cites (Scopus)


Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.

Idioma originalAnglès
Pàgines (de-a)1336-1341
Nombre de pàgines6
RevistaPhotochemical and Photobiological Sciences
Estat de la publicacióPublicada - d’oct. 2010


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