Role of cold ischemia in acute rejection: Characterization of a humoral-like acute rejection in experimental renal transplantation

I. Herrero-Fresneda, M. Franquesa, J. Torras, A. Vidal, J. Aran, R. Pluvinet, N. Lloberas, I. Rama, J. M. Cruzado, O. Gulías, J. M. Grinyó

Producció científica: Article en revista indexadaArticleAvaluat per experts

10 Cites (Scopus)


The aim of the study was to characterize the role of cold ischemia in the process of acute rejection using an experimental renal transplant model. Syngeneic renal transplants were performed between Wistar Agouti rats and allogeneic grafts using Wistar-Agouti rats as recipients of Brown-Norway kidneys. For cold ischemia (CI), kidneys were preserved in Euro-Collins (4°C/ 2.5 hours). Rats were bilaterally nephrectomized at the moment of renal transplant and did not receive any immunosuppressant. The groups were NoAR (n = 6): immediate syngeneic transplant; CI-NoAR (n = 6): syngeneic transplant with CI; AR (n = 13): immediate allogeneic graft; CI-AR (n = 6): allogeneic graft with CI. Allogeneic rats were followed for the survival study. Syngeneic rats, with mean survival time beyond 6 months, were sacrificed on the day 7 to compare grafts with those in the allogeneic groups. H&E- and PAS-stained grafts were evaluated using the Banff criteria. Tissue INF-γ and TNF-α were quantified by RT-real time-PCR on the kidney grafts. Renal insufficiency did not appear in the NoAR group, but it did from the posttransplant day 5 in both acute rejection groups. While NoAR kidneys showed well-conserved renal architecture, then AR group displayed variable degrees of tubular necrosis with scarce cellular infiltration, interstitial hemorrhage, vascular damage with fibrinoid necrosis, perivascular edema, and nuclear disruption. Cold ischemia in rejecting animals increased the mortality rate due to renal insufficiency and accelerated acute rejection. Independently of CI, the proinflammatory cytokines TNF-α and INF-γ were increased in both rejection groups. In conclusion, addition of CI overactivates the acute rejection process via a humoral component.

Idioma originalAnglès
Pàgines (de-a)3712-3715
Nombre de pàgines4
RevistaTransplantation Proceedings
Estat de la publicacióPublicada - de nov. 2005
Publicat externament


Navegar pels temes de recerca de 'Role of cold ischemia in acute rejection: Characterization of a humoral-like acute rejection in experimental renal transplantation'. Junts formen un fingerprint únic.

Com citar-ho