Reactive polymer coatings: A first step toward surface engineering of microfluidic devices

Jörg Lahann, Mercedes Balcells, Hang Lu, Teresa Rodon, Klavs F. Jensen, Robert Langer

Producció científica: Article en revista indexadaArticleAvaluat per experts

171 Cites (Scopus)

Resum

We report fabrication, characterization, and use of micro-fluidic analysis devices containing surface-immobilized cell-capturing molecules. Amino-terminated biotin ligands are immobilized onto the luminal surface of a microdevice and effectively support self-assembly of proteins, antibodies, and mammalian cells. For this purpose, chemical vapor deposition (CVD) polymerization is used to functionalize PDMS-made microfluidic devices with poly-[para-xylylene carboxylic acid pentafluorophenolester-co-para-xylylene]. The resulting reactive coating shows excellent adhesion when deposited in thin films (∼100 nm, and the distribution of the pentafluorophenol ester groups is reasonably uniform within the microchannel inner surface, as examined by fluorescence microscopy. The utility of these devices for cell-based bioassays is demonstrated by monitoring the concentration-dependent effect of the disintegrin echistatin on cell adhesion. The described assay format could be relevant to clinical research in various fields, including angiogenesis research.

Idioma originalAnglès
Pàgines (de-a)2117-2122
Nombre de pàgines6
RevistaAnalytical Chemistry
Volum75
Número9
DOIs
Estat de la publicacióPublicada - 1 de maig 2003

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