TY - JOUR
T1 - Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase
AU - Querol, Enrique
AU - Padrós, Esteve
AU - Juncosa, Miquel
AU - Planas, Antoni
AU - Lloberas, Jorge
N1 - Funding Information:
ACKNOWLEDGMENTS. This work was supported by grants from CICYT (Ministerio of Education y Ciencia) PBT87-31 and 81091-0477 to E.Q. and PB89-0301 to E.P. A. Planas and M. Juncosa are, respectively, recipients of post and pre-doctoral fellowships from Ministerio de Education y Ciencia.
PY - 1992/4/30
Y1 - 1992/4/30
N2 - The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.
AB - The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.
UR - http://www.scopus.com/inward/record.url?scp=0026650411&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(92)90633-V
DO - 10.1016/0006-291X(92)90633-V
M3 - Article
C2 - 1575735
AN - SCOPUS:0026650411
SN - 0006-291X
VL - 184
SP - 612
EP - 617
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -