Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol; Esteve Padrós; Miquel Juncosa; Antoni Planas; Jorge Lloberas

doi:10.1016/0006-291X(92)90633-V

Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol, Esteve Padrós, Miquel Juncosa, Antoni Planas, Jorge Lloberas

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The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.

Idioma original	Anglès
Pàgines (de-a)	612-617
Nombre de pàgines	6
Revista	Biochemical and Biophysical Research Communications
Volum	184
Número	2
DOIs	https://doi.org/10.1016/0006-291X(92)90633-V
Estat de la publicació	Publicada - 30 d’abr. 1992

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@article{4d2acbb50e0c49d983172aad8caf638b,

title = "Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase",

abstract = "The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.",

keywords = "Beta-glucanase, Gene, Proteins, Sequence, Subtilis, Spectra",

author = "Enrique Querol and Esteve Padr{\'o}s and Miquel Juncosa and Antoni Planas and Jorge Lloberas",

year = "1992",

month = apr,

day = "30",

doi = "10.1016/0006-291X(92)90633-V",

language = "English",

volume = "184",

pages = "612--617",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase. / Querol, Enrique; Padrós, Esteve; Juncosa, Miquel et al.
In: Biochemical and Biophysical Research Communications, Vol. 184, Núm. 2, 30.04.1992, pàg. 612-617.

Producció científica: Article en revista indexada › Article › Avaluat per experts

TY - JOUR

T1 - Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

AU - Querol, Enrique

AU - Padrós, Esteve

AU - Juncosa, Miquel

AU - Planas, Antoni

AU - Lloberas, Jorge

PY - 1992/4/30

Y1 - 1992/4/30

N2 - The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.

AB - The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.

KW - Beta-glucanase

KW - Gene

KW - Proteins

KW - Sequence

KW - Subtilis

KW - Spectra

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U2 - 10.1016/0006-291X(92)90633-V

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AN - SCOPUS:0026650411

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

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