TY - JOUR
T1 - Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System
AU - Makhijani, Kalpana
AU - To, Tsz Leung
AU - Ruiz-González, Rubén
AU - Lafaye, Céline
AU - Royant, Antoine
AU - Shu, Xiaokun
N1 - Publisher Copyright:
© 2017
PY - 2017/1/19
Y1 - 2017/1/19
N2 - Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology.
AB - Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology.
KW - cell ablation
KW - developmental biology
KW - Drosophila
KW - fluorescent proteins
KW - neurons
KW - optogenetics
KW - photoreceptor
KW - photosensitizer
KW - reactive oxyge species
KW - wing
UR - http://www.scopus.com/inward/record.url?scp=85008474526&partnerID=8YFLogxK
U2 - 10.1016/j.chembiol.2016.12.010
DO - 10.1016/j.chembiol.2016.12.010
M3 - Article
C2 - 28065655
AN - SCOPUS:85008474526
SN - 2451-9456
VL - 24
SP - 110
EP - 119
JO - Cell Chemical Biology
JF - Cell Chemical Biology
IS - 1
ER -