TY - JOUR
T1 - Powerful combination of analytical and chemometric methods for the photodegradation of 5-Fluorouracil
AU - Gómez-Canela, Cristian
AU - Bolivar-Subirats, Gabino
AU - Tauler, Romà
AU - Lacorte, Silvia
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/4/15
Y1 - 2017/4/15
N2 - The photodegradation of the antineoplastic drug 5-fluorouracil (5-FU) under the action of UV light was studied using UV–vis spectroscopy and High Performance Liquid Chromatography coupled to Diode Array Detector and High Resolution Mass Spectrometry (HPLC-DAD-HRMS). To analyze, integrate and interpret the degradation kinetics, the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) chemometric method has been applied. The high complexity of the photodegradation process of this drug involving up to seven different photoproducts showed the advantages of the proposed approach which provides simultaneously mechanistic and structural information explaining the 5-FU photodegradability. Moreover, this study provides a new tool to be used for elucidating the photodegradation kinetics at real time.
AB - The photodegradation of the antineoplastic drug 5-fluorouracil (5-FU) under the action of UV light was studied using UV–vis spectroscopy and High Performance Liquid Chromatography coupled to Diode Array Detector and High Resolution Mass Spectrometry (HPLC-DAD-HRMS). To analyze, integrate and interpret the degradation kinetics, the Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) chemometric method has been applied. The high complexity of the photodegradation process of this drug involving up to seven different photoproducts showed the advantages of the proposed approach which provides simultaneously mechanistic and structural information explaining the 5-FU photodegradability. Moreover, this study provides a new tool to be used for elucidating the photodegradation kinetics at real time.
KW - 5-Fluorouracil
KW - LC-DAD
KW - LC-HRMS
KW - MCR-ALS
KW - Photodegradation
UR - https://www.scopus.com/pages/publications/85009090868
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000395357100004&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.jpba.2017.01.019
DO - 10.1016/j.jpba.2017.01.019
M3 - Article
C2 - 28088664
AN - SCOPUS:85009090868
SN - 0731-7085
VL - 137
SP - 33
EP - 41
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -