TY - JOUR
T1 - Poly(D, L-lactide-co-glycolide) nanoparticles as delivery agents for photodynamic therapy
T2 - Enhancing singlet oxygen release and photototoxicity by surface PEG coating
AU - Boix-Garriga, Ester
AU - Acedo, Pilar
AU - Casadó, Ana
AU - Villanueva, Angeles
AU - Stockert, Juan Carlos
AU - Cañete, Magdalena
AU - Mora, Margarita
AU - Sagristá, Maria Lluïsa
AU - Nonell, Santi
N1 - Publisher Copyright:
© 2015 IOP Publishing Ltd.
PY - 2015/9/11
Y1 - 2015/9/11
N2 - Poly(D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) are being considered as nanodelivery systems for photodynamic therapy. The physico-chemical and biological aspects of their use remain largely unknown. Herein we report the results of a study of PLGA NPs for the delivery of the model hydrophobic photosensitizer ZnTPP to HeLa cells. ZnTPP was encapsulated in PLGA with high efficiency and the NPs showed negative zeta potentials and diameters close to 110 nm. Poly(ethylene glycol) (PEG) coating, introduced to prevent opsonization and clearance by macrophages, decreased the size and zeta potential of the NPs by roughly a factor of two and improved their stability in the presence of serum proteins. Photophysical studies revealed two and three populations of ZnTPP and singlet oxygen in uncoated and PEGylated NPs, respectively. Singlet oxygen is confined within the NPs in bare PLGA while it is more easily released into the external medium after PEG coating, which contributes to a higher photocytotoxicity towards HeLa cells in vitro. PLGA NPs are internalized by endocytosis, deliver their cargo to lysosomes and induce cell death by apoptosis upon exposure to light. In conclusion, PLGA NPs coated with PEG show high potential as delivery systems for photodynamic applications.
AB - Poly(D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) are being considered as nanodelivery systems for photodynamic therapy. The physico-chemical and biological aspects of their use remain largely unknown. Herein we report the results of a study of PLGA NPs for the delivery of the model hydrophobic photosensitizer ZnTPP to HeLa cells. ZnTPP was encapsulated in PLGA with high efficiency and the NPs showed negative zeta potentials and diameters close to 110 nm. Poly(ethylene glycol) (PEG) coating, introduced to prevent opsonization and clearance by macrophages, decreased the size and zeta potential of the NPs by roughly a factor of two and improved their stability in the presence of serum proteins. Photophysical studies revealed two and three populations of ZnTPP and singlet oxygen in uncoated and PEGylated NPs, respectively. Singlet oxygen is confined within the NPs in bare PLGA while it is more easily released into the external medium after PEG coating, which contributes to a higher photocytotoxicity towards HeLa cells in vitro. PLGA NPs are internalized by endocytosis, deliver their cargo to lysosomes and induce cell death by apoptosis upon exposure to light. In conclusion, PLGA NPs coated with PEG show high potential as delivery systems for photodynamic applications.
KW - PLGA
KW - nanoparticle
KW - photodynamic therapy
KW - photosensitizer
KW - polyethyleneglycol
KW - singlet oxygen
UR - http://www.scopus.com/inward/record.url?scp=84940100250&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000360973400005&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1088/0957-4484/26/36/365104
DO - 10.1088/0957-4484/26/36/365104
M3 - Article
C2 - 26293792
AN - SCOPUS:84940100250
SN - 0957-4484
VL - 26
JO - Nanotechnology
JF - Nanotechnology
IS - 36
M1 - 365104
ER -