TY - JOUR
T1 - PLGA cationic nanoparticles, obtained from nano-emulsion templating, as potential DNA vaccines
AU - Soler Besumbes, Eduard
AU - Fornaguera, Cristina
AU - Monge, Marta
AU - García-Celma, María José
AU - Carrión, Javier
AU - Solans, Conxita
AU - Dols-Perez, Aurora
N1 - Funding Information:
Financial support from MINECO (grants CTQ2016-80645-R and CTQ2017-84998-P); Generalitat de Catalunya (grants 2014SGR1655 and 2017SGR1778), and CIBER-BBN are acknowledged. CIBER-BBN is an initiative funded by the Spanish National Plan for Scientific and Technical Research and Innovation 2013–2016, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. Dr. Cristina Fornaguera is grateful to AGAUR for their Predoctoral Fellowship (grant FI-DGR 2012) and to MINECO for their Torres Quevedo Postdoctoral Fellowship(grant PTQ 2015). Authors thank the access to the facilities of TEM-SEM Casanova unit of the Centres Científics i Tecnològics (CCiTUB) of the University of Barcelona (UB) and its staff members for their technical advice.
Funding Information:
Financial support from MINECO (grants CTQ2016-80645-R and CTQ2017-84998-P ); Generalitat de Catalunya (grants 2014SGR1655 and 2017SGR1778 ), and CIBER-BBN are acknowledged. CIBER-BBN is an initiative funded by the Spanish National Plan for Scientific and Technical Research and Innovation 2013–2016, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund . Dr. Cristina Fornaguera is grateful to AGAUR for their Predoctoral Fellowship (grant FI-DGR 2012 ) and to MINECO for their Torres Quevedo Postdoctoral Fellowship (grant PTQ 2015 ). Authors thank the access to the facilities of TEM-SEM Casanova unit of the Centres Científics i Tecnològics (CCiTUB) of the University of Barcelona (UB) and its staff members for their technical advice.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/11
Y1 - 2019/11
N2 - Polymeric nanoparticles offer advantageous characteristics as gene-delivery vectors such as biocompatibility and biodegradability. With this aim, a smart and innovative strategy was followed here: Cationic PLGA nano-emulsions, prepared by a low energy method, were used as templates to obtain cationic nanoparticles (NPs) able to easily complex with nucleic acids (i.e. plasmid DNA) by electrostatic interactions. The strategy employed to produce stable positively-charged nanoparticles was the use of non-ionic/cationic surfactant mixtures to stabilize template nano-emulsions. This methodology allowed obtaining nanoparticles with reproducible nanometric sizes and positive zeta potential values, appropriate to successfully complex with nucleic acids, resulting in nanometric spherical polyplexes. Nanoparticles, plasmids and polyplexes proved to be biocompatible at the optimal concentration. Therefore, we can conclude that we have designed a novel strategy to efficiently obtain cationic polymeric nanoparticles that can be a promising approach to act as novel non-viral gene-delivery vectors, useful for many applications in gene therapy, such as gene vaccines.
AB - Polymeric nanoparticles offer advantageous characteristics as gene-delivery vectors such as biocompatibility and biodegradability. With this aim, a smart and innovative strategy was followed here: Cationic PLGA nano-emulsions, prepared by a low energy method, were used as templates to obtain cationic nanoparticles (NPs) able to easily complex with nucleic acids (i.e. plasmid DNA) by electrostatic interactions. The strategy employed to produce stable positively-charged nanoparticles was the use of non-ionic/cationic surfactant mixtures to stabilize template nano-emulsions. This methodology allowed obtaining nanoparticles with reproducible nanometric sizes and positive zeta potential values, appropriate to successfully complex with nucleic acids, resulting in nanometric spherical polyplexes. Nanoparticles, plasmids and polyplexes proved to be biocompatible at the optimal concentration. Therefore, we can conclude that we have designed a novel strategy to efficiently obtain cationic polymeric nanoparticles that can be a promising approach to act as novel non-viral gene-delivery vectors, useful for many applications in gene therapy, such as gene vaccines.
KW - Gene-delivery vector
KW - Nano-emulsion templating
KW - Non-viral gene therapy
KW - Polymeric nanoparticles
KW - Polyplexes
UR - http://www.scopus.com/inward/record.url?scp=85072166603&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000498309000035&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.eurpolymj.2019.109229
DO - 10.1016/j.eurpolymj.2019.109229
M3 - Article
AN - SCOPUS:85072166603
SN - 0014-3057
VL - 120
JO - European Polymer Journal
JF - European Polymer Journal
M1 - 109229
ER -