PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

P. Vincent; L. R. V. Castro; N. Gervasi; E. Guiot; M. Brito; D. Paupardin-Tritsch

doi:10.1055/s-0032-1311631

PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

P. Vincent, L. R. V. Castro, N. Gervasi, E. Guiot, M. Brito, D. Paupardin-Tritsch

Producció científica: Article en revista indexada › Article › Avaluat per experts

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Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.

Idioma original	Anglès
Pàgines (de-a)	786-789
Nombre de pàgines	4
Revista	Hormone and Metabolic Research
Volum	44
Número	10
DOIs	https://doi.org/10.1055/s-0032-1311631
Estat de la publicació	Publicada - de set. 2012
Publicat externament	Sí

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title = "PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons",

abstract = "Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.",

keywords = "Cortex, cyclic AMP, Imaging, Neuron, Phosphodiesterase, Protein kinase",

author = "P. Vincent and Castro, {L. R. V.} and N. Gervasi and E. Guiot and M. Brito and D. Paupardin-Tritsch",

year = "2012",

month = sep,

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T1 - PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

AU - Vincent, P.

AU - Castro, L. R. V.

AU - Gervasi, N.

AU - Guiot, E.

AU - Brito, M.

AU - Paupardin-Tritsch, D.

PY - 2012/9

Y1 - 2012/9

N2 - Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.

AB - Electrophysiological recordings (using the slow-AHP potassium current) together with novel biosensor imaging methods (with AKAR and Epac sensors) were used in preparations of rodent brain slices to record PKA activation in real time and in individual neurons. The experiments revealed the propagation of the PKA signal from the membrane to the cytosol and eventually to the nucleus. The experiments show how the geometry of the neurons combined with phosphodiesterase activities (mostly rolipram-sensitive PDE4) contributes to a functional compartmentation of the cAMP in subcellular domains.

KW - Cortex

KW - cyclic AMP

KW - Imaging

KW - Neuron

KW - Phosphodiesterase

KW - Protein kinase

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DO - 10.1055/s-0032-1311631

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SN - 0018-5043

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EP - 789

JO - Hormone and Metabolic Research

JF - Hormone and Metabolic Research

IS - 10

ER -

PDE4 Control on cAMP/PKA Compartmentation Revealed by Biosensor Imaging in Neurons

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