TY - JOUR
T1 - Optimizing the properties of the protein corona surrounding nanoparticles for tuning payload release
AU - Cifuentes-Rius, Anna
AU - De Puig, Helena
AU - Kah, James Chen Yong
AU - Borros, Salvador
AU - Hamad-Schifferli, Kimberly
PY - 2013/11/26
Y1 - 2013/11/26
N2 - We manipulate the passive release rates of DNA payloads on protein coronas formed around nanoparticles (NPs) by varying the corona composition. The coronas are prepared using a mixture of hard and soft corona proteins. We form coronas around gold nanorods (NRs), nanobones (NBs), and carbon nanotubes (CNTs) from human serum (HS) and find that tuning the amount of human serum albumin (HSA) in the NR-coronas (NR-HS-DNA) changes the payload release profile. The effect of buffer strength, HS concentration, and concentration of the cetyltrimethylammonium bromide (CTAB) passivating the NP surfaces on passive release is explored. We find that corona properties play an important role in passive release, and concentrations of CTAB, HS, and phosphate buffer used in corona formation can tune payload release profiles. These advances in understanding protein corona properties bring us closer toward developing a set of basic design rules that enable their manipulation and optimization for particular biological applications.
AB - We manipulate the passive release rates of DNA payloads on protein coronas formed around nanoparticles (NPs) by varying the corona composition. The coronas are prepared using a mixture of hard and soft corona proteins. We form coronas around gold nanorods (NRs), nanobones (NBs), and carbon nanotubes (CNTs) from human serum (HS) and find that tuning the amount of human serum albumin (HSA) in the NR-coronas (NR-HS-DNA) changes the payload release profile. The effect of buffer strength, HS concentration, and concentration of the cetyltrimethylammonium bromide (CTAB) passivating the NP surfaces on passive release is explored. We find that corona properties play an important role in passive release, and concentrations of CTAB, HS, and phosphate buffer used in corona formation can tune payload release profiles. These advances in understanding protein corona properties bring us closer toward developing a set of basic design rules that enable their manipulation and optimization for particular biological applications.
KW - carbon nanotubes
KW - controlled release DNA
KW - nanobones
KW - nanorods
KW - protein corona
UR - http://www.scopus.com/inward/record.url?scp=84888878241&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000327752200054&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1021/nn404166q
DO - 10.1021/nn404166q
M3 - Article
C2 - 24128271
AN - SCOPUS:84888878241
SN - 1936-0851
VL - 7
SP - 10066
EP - 10074
JO - ACS Nano
JF - ACS Nano
IS - 11
ER -