TY - JOUR
T1 - Optimal design for studying mucoadhesive polymers interaction with gastric mucin using a quartz crystal microbalance with dissipation (QCM-D)
T2 - Comparison of two different mucin origins
AU - Oh, Sejin
AU - Wilcox, Matthew
AU - Pearson, Jeffrey P.
AU - Borrós, Salvador
N1 - Funding Information:
We thank EU European Project, Alexander, under the Seventh European Community Framework Programme (Grant Agreement Number 280761) for their financial supports of this research.
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - The objective of this present study was to develop an efficient and simple method, based on the use of a quartz crystal microbalance with dissipation (QCM-D), to evaluate the mucoadhesive characteristics of cationic polymers; chitosan, thiolated chitosan (chitosan-SH), and polyallylamine hydrochloride (PAH), and anionic polymers; hyaluronic acid (HA) and thiolated hyaluronic acid (HA-SH). The experiments were carried out at pH 4 to assess the interaction between mucoadhesive polymers and a mucin-coated gold surface. A key point in the QCM-D protocol development was to evaluate two sources of mucin: native porcine gastric mucin (NPGM) and commercially available porcine gastric mucin (CPGM). QCM-D has shown its potential as a highly sensitive technique that provides information about the interaction of mucoadhesive polymers with gastric mucin. The technique would allow the classification of these polymers in order to further assess their application as base materials for nanocarriers, designed to interact with the mucosal barrier which represents a stumbling block for drug adsorption.
AB - The objective of this present study was to develop an efficient and simple method, based on the use of a quartz crystal microbalance with dissipation (QCM-D), to evaluate the mucoadhesive characteristics of cationic polymers; chitosan, thiolated chitosan (chitosan-SH), and polyallylamine hydrochloride (PAH), and anionic polymers; hyaluronic acid (HA) and thiolated hyaluronic acid (HA-SH). The experiments were carried out at pH 4 to assess the interaction between mucoadhesive polymers and a mucin-coated gold surface. A key point in the QCM-D protocol development was to evaluate two sources of mucin: native porcine gastric mucin (NPGM) and commercially available porcine gastric mucin (CPGM). QCM-D has shown its potential as a highly sensitive technique that provides information about the interaction of mucoadhesive polymers with gastric mucin. The technique would allow the classification of these polymers in order to further assess their application as base materials for nanocarriers, designed to interact with the mucosal barrier which represents a stumbling block for drug adsorption.
KW - Adsorption
KW - CPGM
KW - Interaction
KW - Mucin
KW - Mucoadhesive polymers
KW - NPGM
KW - QCM-D
UR - http://www.scopus.com/inward/record.url?scp=84946725856&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000364881900048&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.ejpb.2015.08.002
DO - 10.1016/j.ejpb.2015.08.002
M3 - Article
C2 - 26272125
AN - SCOPUS:84946725856
SN - 0939-6411
VL - 96
SP - 477
EP - 483
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
ER -