TY - JOUR
T1 - Online monitoring of myocardial bioprosthesis for cardiac repair
AU - Prat-Vidal, Cristina
AU - Gálvez-Montón, Carolina
AU - Puig-Sanvicens, Verónica
AU - Sanchez, Benjamin
AU - Díaz-Güemes, Idoia
AU - Bogónez-Franco, Paco
AU - Perea-Gil, Isaac
AU - Casas-Solà, Anna
AU - Roura, Santiago
AU - Llucià-Valldeperas, Aida
AU - Soler-Botija, Carolina
AU - Sánchez-Margallo, Francisco M.
AU - Semino, Carlos E.
AU - Bragos, Ramon
AU - Bayes-Genis, Antoni
N1 - Funding Information:
Grant support : This work was supported in part by the Instituto de Salud Carlos III: Redes Temáticas de Investigación Cooperativa en Salud (Red de Investigación Cardiovascular [RIC, RD12/0042/0047 and Red de Investigación en Terapia Celular–TerCel, RD12/0019/0029] and Infrastructure Grant [IF09/3667]; by the Ministerio de Ciencia e Innovación [SAF2011-30067-C02-01/02 to A.B.G.]; and from Fundació Privada Daniel Bravo Andreu, and La Marató de TV3 [080330 to A.B.G. and 12/2232 to S.R.].
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Background/objectives The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized. Methods Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n = 8 treated and n = 5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections. Results Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67+-GFP+-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin+-GFP+-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation. Conclusions With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
AB - Background/objectives The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized. Methods Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n = 8 treated and n = 5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections. Results Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67+-GFP+-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin+-GFP+-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation. Conclusions With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
KW - Adipose tissue derived progenitor cells
KW - Decellularized pericardium
KW - Myocardial bioprosthesis
KW - Myocardial infarction
KW - Swine model
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UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000339261400043&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.ijcard.2014.04.181
DO - 10.1016/j.ijcard.2014.04.181
M3 - Article
C2 - 24820760
AN - SCOPUS:84903277632
SN - 0167-5273
VL - 174
SP - 654
EP - 661
JO - International Journal of Cardiology
JF - International Journal of Cardiology
IS - 3
ER -