TY - JOUR
T1 - On the mechanism of Candida spp. photoinactivation by hypericin
AU - López-Chicón, Patricia
AU - Paz-Cristobal, Manuel P.
AU - Rezusta, Antonio
AU - Aspiroz, Carmen
AU - Royo-Cañas, María
AU - Andres-Ciriano, Elena
AU - Gilaberte, Yolanda
AU - Agut, Montserrat
AU - Nonell, Santi
N1 - Publisher Copyright:
© The Royal Society of Chemistry and Owner Societies 2012.
PY - 2012/6/1
Y1 - 2012/6/1
N2 - The photoprocesses involved in hypericin photoinactivation of three different Candida species (C. albicans, C. parapsilosis and C. krusei) have been examined. Production of singlet oxygen from the triplet state and of superoxide from both the triplet state and the semiquinone radical anion are demonstrated. Hydrogen peroxide is formed downstream of these early events. The outcome of the photodynamic treatments is dictated by the intracellular distribution of hypericin, which is different in the three species and affects the ability of hypericin to produce the different reactive oxygen species and trigger cell-death pathways. The results are in line with the previously-observed different susceptibilities of the three Candida species to hypericin photodynamic treatments.
AB - The photoprocesses involved in hypericin photoinactivation of three different Candida species (C. albicans, C. parapsilosis and C. krusei) have been examined. Production of singlet oxygen from the triplet state and of superoxide from both the triplet state and the semiquinone radical anion are demonstrated. Hydrogen peroxide is formed downstream of these early events. The outcome of the photodynamic treatments is dictated by the intracellular distribution of hypericin, which is different in the three species and affects the ability of hypericin to produce the different reactive oxygen species and trigger cell-death pathways. The results are in line with the previously-observed different susceptibilities of the three Candida species to hypericin photodynamic treatments.
UR - http://www.scopus.com/inward/record.url?scp=84866447390&partnerID=8YFLogxK
U2 - 10.1039/c2pp25105a
DO - 10.1039/c2pp25105a
M3 - Article
C2 - 22566080
AN - SCOPUS:84866447390
SN - 1474-905X
VL - 11
SP - 1099
EP - 1107
JO - Photochemical and Photobiological Sciences
JF - Photochemical and Photobiological Sciences
IS - 6
ER -