TY - JOUR
T1 - On-line monitoring of yeast cell growth by impedance spectroscopy
AU - Soley, A.
AU - Lecina, M.
AU - Gámez, X.
AU - Cairó, J. J.
AU - Riu, P.
AU - Rosell, X.
AU - Bragós, R.
AU - Gòdia, F.
PY - 2005/9/10
Y1 - 2005/9/10
N2 - The application of impedance spectroscopy to estimate on-line cell concentration was studied. The estimation was based on the relative variation between electrical impedance measured at low (10 kHz) and high frequencies (10 MHz). Studies were carried out to characterise the influence of changes in physical and chemical parameters on the impedance measurement. Two different possibilities to perform on-line measurements were tested: a simple set-up, based on an in situ probe, gave good results but was not suitable for high agitation and aeration rates. An ex situ flow-through on-line measuring cell was used to overcome these problems, showing a better performance. The use of this set-up for the growth monitorisation of a Saccharomyces cerevisiae culture showed an efficient performance, having the correlation between estimated and measured S. cerevisiae a Pearson coefficient of 0.999.
AB - The application of impedance spectroscopy to estimate on-line cell concentration was studied. The estimation was based on the relative variation between electrical impedance measured at low (10 kHz) and high frequencies (10 MHz). Studies were carried out to characterise the influence of changes in physical and chemical parameters on the impedance measurement. Two different possibilities to perform on-line measurements were tested: a simple set-up, based on an in situ probe, gave good results but was not suitable for high agitation and aeration rates. An ex situ flow-through on-line measuring cell was used to overcome these problems, showing a better performance. The use of this set-up for the growth monitorisation of a Saccharomyces cerevisiae culture showed an efficient performance, having the correlation between estimated and measured S. cerevisiae a Pearson coefficient of 0.999.
KW - Biomass estimator
KW - Impedance spectroscopy
KW - On-line monitoring
UR - http://www.scopus.com/inward/record.url?scp=23044511479&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000231302300007&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.jbiotec.2005.05.022
DO - 10.1016/j.jbiotec.2005.05.022
M3 - Article
C2 - 16026878
AN - SCOPUS:23044511479
SN - 0168-1656
VL - 118
SP - 398
EP - 405
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 4
ER -