TY - JOUR
T1 - Methyl methacrylate modified chitosan
T2 - Synthesis, characterization and application in drug and gene delivery
AU - Jaiswal, Shefali
AU - Dutta, P. K.
AU - Kumar, Santosh
AU - Koh, Joonseok
AU - Pandey, Shambhavi
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/5/1
Y1 - 2019/5/1
N2 - A methyl methacrylate (MMA) modified chitosan (CS) conjugate (CSMMA) has been synthesized by a green method via Michael addition reaction between CS and MMA in ethanol. The synthesized conjugate was characterized by FT-IR, 1 H NMR, X-ray diffraction spectrometry and SEM analysis. The results confirmed that CS was covalently linked to MMA yielding a highly porous framework. The uses of CSMMA were analyzed as a potential gene and drug delivery agent. CSMMA proved to be a reasonably good gene delivery agent based on transfection efficiency studies in mammalian cancer cell lines (A549, HeLa and HepG2). For drug delivery studies, nanoparticles of the CSMMA biopolymer were prepared by ionic gelation method with sodium tripolyphosphate (TPP). The prepared nanoparticles were characterized in terms of FE-SEM, DLS and zeta potential. In vitro drug release study of curcumin loaded CSMMA nanoparticles showed its maximal entrapment efficiency up to 68% and the drug release was more rapid at a pH (5.0) lower than physiological pH.
AB - A methyl methacrylate (MMA) modified chitosan (CS) conjugate (CSMMA) has been synthesized by a green method via Michael addition reaction between CS and MMA in ethanol. The synthesized conjugate was characterized by FT-IR, 1 H NMR, X-ray diffraction spectrometry and SEM analysis. The results confirmed that CS was covalently linked to MMA yielding a highly porous framework. The uses of CSMMA were analyzed as a potential gene and drug delivery agent. CSMMA proved to be a reasonably good gene delivery agent based on transfection efficiency studies in mammalian cancer cell lines (A549, HeLa and HepG2). For drug delivery studies, nanoparticles of the CSMMA biopolymer were prepared by ionic gelation method with sodium tripolyphosphate (TPP). The prepared nanoparticles were characterized in terms of FE-SEM, DLS and zeta potential. In vitro drug release study of curcumin loaded CSMMA nanoparticles showed its maximal entrapment efficiency up to 68% and the drug release was more rapid at a pH (5.0) lower than physiological pH.
KW - Chitosan
KW - Drug delivery
KW - Gene delivery
KW - Methyl methacrylate (MMA)
KW - Nanoparticle
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UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000459866600013&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.carbpol.2019.01.104
DO - 10.1016/j.carbpol.2019.01.104
M3 - Article
C2 - 30824069
AN - SCOPUS:85061059265
SN - 0144-8617
VL - 211
SP - 109
EP - 117
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -
