Mechanical double layer model for Saccharomyces Cerevisiae cell wall

Ruben Mercadé-Prieto, Colin R. Thomas, Zhibing Zhang

Producció científica: Article en revista indexadaArticleAvaluat per experts

16 Cites (Scopus)

Resum

The elastic modulus of the Baker's yeast (Saccharomyces cerevisiae) cell wall reported in studies using atomic force microscopy (AFM) is two orders of magnitude lower than that obtained using whole cell compression by micromanipulation. Using finite element modelling, it is shown that Hertz-Sneddon analysis cannot be applied to AFM indentation data for single layer core-shell structures. In addition, the Reissner solution for shallow homogeneous spheres is not appropriate for thick walls such as those of yeast cells. In order to explain yeast compression measurements at different length scales, a double layer wall model is presented considering a soft external layer composed of mannoproteins, and a stiff inner layer of β-glucan fibres and chitin. Under this model, previous AFM studies using sharp indenters provide reasonable estimates of the external layer elastic modulus, while micromanipulation provides the total stiffness of the cell wall. Data from both measurements are combined to estimate the mechanical properties of the inner stiff layer.

Idioma originalAnglès
Pàgines (de-a)613-620
Nombre de pàgines8
RevistaEuropean Biophysics Journal
Volum42
Número8
DOIs
Estat de la publicacióPublicada - d’ag. 2013
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