Liposcale: A novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy

Roger Mallol, Núria Amigó, Miguel A. Rodríguez, Mercedes Heras, Maria Vinaixa, Núria Plana, Edmond Rock, Josep Ribalta, Oscar Yanes, Lluís Masana, Xavier Correig

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124 Cites (Scopus)

Resum

Determination of lipoprotein particle size and number using advanced lipoprotein tests (ALTs) is of particular importance to improve cardiovascular risk prediction. Here we present the Liposcale test, a novel ALT based on 2D diffusion-ordered 1H NMR spectroscopy. Our method uses diffusion coefficients to provide a direct measure of the mean particle sizes and numbers. Using 177 plasma samples from healthy individuals and the concentration of ApoB and ApoA from isolated lipoprotein fractions, our test showed a stronger correlation between the NMR-derived lipoprotein particle numbers and apolipoprotein concentrations than the LipoProfile ® test commercialized by Liposcience. We also converted LDL particle numbers to ApoB equivalents (milligrams per deciliter) and our test yielded similar values of LDL-ApoB to the LipoProfile ® test (absolute mean bias of 8.5 and 7.4 mg/dl, respectively). In addition, our HDL particle number values were more concordant with the calibrated values determined recently using ion mobility. Finally, principal component analysis distinguished type 2 diabetic patients with and without atherogenic dyslipidemia (AD) on a second cohort of 307 subjects characterized using the Liposcale test (area under the curve = 0.88) and showed concordant relationships between variables explaining AD. Altogether, our method provides reproducible and reliable characterization of lipoprotein particles and it is applicable to pathological states such as AD.

Idioma originalAnglès
Pàgines (de-a)737-746
Nombre de pàgines10
RevistaJournal of Lipid Research
Volum56
Número3
DOIs
Estat de la publicacióPublicada - 1 de març 2015
Publicat externament

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