Lipase activity and enantioselectivity of whole cells from a wild-type Aspergillius flavus strain

Carmen Solarte, Edinson Yara-Varón, Jordi Eras, Mercè Torres, Mercè Balcells, Ramon Canela-Garayoa

Producció científica: Article en revista indexadaArticleAvaluat per experts

15 Cites (Scopus)

Resum

This study reports the high enantiomeric preference of whole cell lipase from Aspergillus flavus wild-type that allows the preparation of a chiral secondary alcohol. Whole cells prepared from a wild-type Aspergillus flavus strain were used as biocatalysts to prepare (R)-1-phenylethyl acetate. (R)-1-Phenylethanol was esterified into (R)-1-phenylethyl acetate with a 94.6% enantiomeric excess (ee) within 24 h at 40 C and (S)-1-phenylethanol remained in the reaction medium with a >99%ee. Besides, this biocatalyst allows the preparation of ethyl laurate and a mixture of 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate. The ethyl laurate yield was 96%, whereas the synthesis of a mixture of the acrylate regioisomers, 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate gave similar yields to those obtained using commercial lipases.

Idioma originalAnglès
Pàgines (de-a)78-83
Nombre de pàgines6
RevistaJournal of Molecular Catalysis B: Enzymatic
Volum100
DOIs
Estat de la publicacióPublicada - de febr. 2014
Publicat externament

Fingerprint

Navegar pels temes de recerca de 'Lipase activity and enantioselectivity of whole cells from a wild-type Aspergillius flavus strain'. Junts formen un fingerprint únic.

Com citar-ho