Immobilized Pichia pastoris in alginate beads for continuous culture

Patricia K. Carvajal-Vallejos, Antoni Planas, Eduard Barberà

Producció científica: Article en revista indexadaArticleAvaluat per experts


Conditions for immobilizing Pichia pastoris GS115pPIC9g by calcium alginate gel entrapment were determined. After mixture immobilization with 1.5 × 109 CFU/ml, free biomass concentration decreases from 2.3 × 104 to 2.2 × 103 CFU/ml when alginate concentration rises from 20 to 40 g/l. Bead diameter with cells was 3.08 ±0.18 mm. Immobilized biomass reutilization power was demonstrated with repeated replacement cultures. Glycerol uptake in a continuous culture with 100 ml of immobilized biomass with 500 ml of medium (36.3 g/l glycerol), reached the stationary state the third day on 52.9 ±1.5 g/d, with a dilution rate of 0.12 h-1. Biomass concentration at stationary phase was 10 7-108 CFU/ml for free cells and 109 CFU/ml for immobilized ones. The distribution pattern of entrapped cells in alginate beads is showed by scanning electron microscopy (SEM) and the morphology of free and immobilized cells by optical microscopy.

Idioma originalAnglès
Pàgines (de-a)384-389
Nombre de pàgines6
Estat de la publicacióPublicada - de maig 2007


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