Identification of Plasma Biomarkers of Human Intracerebral Hemorrhage Subtypes through Microarray Technology

Cristina Merino-Zamorano, Pilar Delgado, Sofía Fernández De Retana, Israel Fernández-Cadenas, David Rodríguez-Luna, Joan Montaner, Mar Hernández-Guillamon

Producció científica: Article en revista indexadaArticleAvaluat per experts

4 Cites (Scopus)


Background Intracerebral hemorrhage (ICH) is a devastating form of stroke and depending on the underlying cause, primary ICH is mainly caused by hypertension (HTN-ICH) or cerebral amyloid angiopathy (CAA-ICH). Currently, neuroimaging markers are required to identify the pattern for each etiology. The discovery of new biomarkers to improve the management of this pathology is therefore needed. Methods A microarray analysis was carried out to analyze gene expression differences in blood samples from patients (>1.5 months since the last ICH event) who suffered a CAA-ICH and HTN-ICH, and controls. The results were replicated by quantitative polymerase chain reaction and the plasma protein level of the best candidate was measured with enzyme-linked immunosorbent assay. Results The microarray analysis and the validation study revealed an increase in Golgin A8 Family, Member A (GOLGA8A) mRNA and protein levels in ICH cases compared to controls (P < .01), although no differences were found between specific ICH etiologies. GOLGA8A plasma levels were also associated with the presence of multiple hemorrhages (P < .05). Conclusions The GOLGA8A level was increased in the blood of patients who suffered a primary ICH. We did not, however, find any candidate biomarker that distinguished CAA-ICH from HTN-ICH. The role of GOLGA8A in this fatal disorder has yet to be determined.

Idioma originalAnglès
Pàgines (de-a)665-671
Nombre de pàgines7
RevistaJournal of Stroke and Cerebrovascular Diseases
Estat de la publicacióPublicada - 1 de març 2016
Publicat externament


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