Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro

Elsa Genové, Stephanie Schmitmeier, Ana Sala, Salvador Borrós, Augustinus Bader, Linda G. Griffith, Carlos E. Semino

Producció científica: Article en revista indexadaArticleAvaluat per experts

53 Cites (Scopus)


There is a major challenge in maintaining functional hepatocytes in vivo as these cells rapidly lose their metabolic properties in culture. In this work we have developed a bioengineered platform that replaces the use of the collagen I - in the traditional culture sandwich technique - by a defined extracellular matrix analogue, the self-assembling peptide hydrogel RAD16-I functionalized with biologically active motifs. Thus, after examining side by side the two culture systems we have found that in both cases hepatocytes acquired similar parenchymal morphology, presence of functional bile canaliculi structures, CYP3A2 induction by dexamethasone, urea production, secretion of proteins such as apolipoprotein (class A1, E, J), α 1-microglobulin, α 1-macroglobulin, retinol binding protein, fibronectin, α 1-inhibitor III and biotin-dependent carboxylases. Interestingly, by assessing in more detail some other hepatic markers, one of the functionalized matrix analogues - carrying the 67 kD laminin receptor ligand - enhanced the gene expression of albumin, HNF4-α, MDR2 and tyrosine aminotransferase. We conclude that the use of a synthetic culture system with designed matrix functionalization has the advantage in controlling specific cellular responses.

Idioma originalAnglès
Pàgines (de-a)3387-3397
Nombre de pàgines11
RevistaJournal of Cellular and Molecular Medicine
Número9 B
Estat de la publicacióPublicada - de set. 2009


Navegar pels temes de recerca de 'Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro'. Junts formen un fingerprint únic.

Com citar-ho